Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Vibrio sp. |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
intracellular | - |
Vibrio sp. | 5622 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Cs+ | 0.2 M, 2500% of initial activity | Vibrio sp. | |
K+ | 0.2 M, 2500% of initial activity | Vibrio sp. | |
Li+ | 0.2 M, 1880% of initial activity | Vibrio sp. | |
Na+ | 0.2 M, 2200% of initial activity | Vibrio sp. | |
NH4+ | 0.2 M, 2270% of initial activity | Vibrio sp. | |
Rb+ | 0.2 M, 2500% of initial activity | Vibrio sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
25009 | - |
x * 25009, calculated | Vibrio sp. |
28000 | - |
1 * 29643, calculated, 1 * 28000, SDS-PAGE | Vibrio sp. |
29643 | - |
1 * 29643, calculated, 1 * 28000, SDS-PAGE | Vibrio sp. |
31000 | - |
gel filtration | Vibrio sp. |
54426 | - |
x * 54426, calculated | Vibrio sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Vibrio sp. | C4TJD2 | sequence contains a putative signal sequence of 23 amino acids | - |
Vibrio sp. | C4TJD3 | - |
- |
Vibrio sp. | C4TJD4 | sequence contains a putative 26-amino-acid signal peptide | - |
Vibrio sp. A9m | C4TJD2 | sequence contains a putative signal sequence of 23 amino acids | - |
Vibrio sp. A9m | C4TJD3 | - |
- |
Vibrio sp. A9m | C4TJD4 | sequence contains a putative 26-amino-acid signal peptide | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
proteolytic modification | sequence contains a putative 26-amino-acid signal peptide | Vibrio sp. |
proteolytic modification | sequence contains a putative signal sequence of 23 amino acids | Vibrio sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1401 | - |
pH 7.5, 30°C | Vibrio sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
alpha-L-guluronosyl linkage in alginate | preferably degrades G blocks | Vibrio sp. | ? | - |
? | |
alpha-L-guluronosyl linkage in alginate | preferably degrades the M block to the G block in alginate | Vibrio sp. | ? | - |
? | |
alpha-L-guluronosyl linkage in alginate | preferably degrades the M block to the G block in alginate | Vibrio sp. A9m | ? | - |
? | |
alpha-L-guluronosyl linkage in alginate | preferably degrades G blocks | Vibrio sp. A9m | ? | - |
? | |
additional information | the relative activities for alginate, M, G, and GM blocks are 100%, 75%, 21%, and 15%, respectively | Vibrio sp. | ? | - |
? | |
additional information | the relative activities for alginate, M, G, and GM blocks are 100%, 75%, 21%, and 15%, respectively | Vibrio sp. A9m | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 25009, calculated | Vibrio sp. |
? | x * 54426, calculated | Vibrio sp. |
monomer | 1 * 29643, calculated, 1 * 28000, SDS-PAGE | Vibrio sp. |
Synonyms | Comment | Organism |
---|---|---|
A9mC | - |
Vibrio sp. |
A9mL | - |
Vibrio sp. |
A9mT | - |
Vibrio sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.6 | 9 | - |
Vibrio sp. |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
8 | 9 | - |
Vibrio sp. |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Vibrio sp. | calculated | - |
4.7 |
Vibrio sp. | calculated | - |
4.9 |
Vibrio sp. | calculated | - |
7.2 |