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Literature summary for 4.2.2.11 extracted from

  • Iwamoto, Y.; Araki, R.; Iriyama, K.; Oda, T.; Fukuda, H.; Hayashida, S.; Muramatsu, T.
    Purification and characterization of bifunctional alginate lyase from Alteromonas sp. strain no. 272 and its action on saturated oligomeric substrates (2001), Biosci. Biotechnol. Biochem., 65, 133-142.
    View publication on PubMed
Search for more literature for 4.2.2.11

General Stability

General Stability Organism
more labile to heat treatment in Tris-HCl buffer, pH 7.0 than in phosphate buffer with similar pH Alteromonas sp.

Inhibitors

Inhibitors Comment Organism Structure
Ba(OH)2 slightly inhibitory, percentage of inhibition is dependent on substrate Alteromonas sp.
NiSO4 slightly inhibitory, percentage of inhibition is dependent on substrate Alteromonas sp.
ZnCl2 slightly inhibitory, percentage of inhibition is dependent on substrate Alteromonas sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.192
-
 saturated hepta((1-4)-alpha-L-guluronan) pH 8.5, 30 °C, 100 mM glycine in 0.1 N NaCl and 0.1 N NaOH Alteromonas sp.
0.226
-
 saturated hexa((1-4)-alpha-L-guluronan) pH 8.5, 30 °C, 100 mM glycine in 0.1 N NaCl and 0.1 N NaOH Alteromonas sp.
0.232
-
 saturated penta((1-4)-alpha-L-guluronan) pH 8.5, 30 °C, 100 mM glycine in 0.1 N NaCl and 0.1 N NaOH Alteromonas sp.
4.25
-
 saturated tetra((1-4)-alpha-L-guluronan) pH 8.5, 30 °C, 100 mM glycine in 0.1 N NaCl and 0.1 N NaOH Alteromonas sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
23000
-
 gel filtration Alteromonas sp.
33900
-
 x * 33900, SDS-PAGE Alteromonas sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
poly-alpha-L-guluronic acid Alteromonas sp.
-
 unsaturated 1,4-di-, 1,4-tri- and 1,4-tetrasaccharides of L-guluronate
-
 ?

Organism

Organism UniProt Comment Textmining
Alteromonas sp.
-
 strain No. 272
-
Alteromonas sp. 272
-
 strain No. 272
-

Purification (Commentary)

Purification (Comment) Organism
anion exchange chromatography, and Sephadex G-100 chromatography Alteromonas sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alpha-L-guluronosyl linkage in alginate endolyase Alteromonas sp. ?
-
 ?
additional information no action on trimeric guluronan and mannuronan, but on tetramers or more, the enzyme is most likely beta-structure, the subsite number is most likely six for both guluronate and mannuronate units, the catalytic site of this enzyme is located at the midpoint of the subsite Alteromonas sp. ?
-
 ?
poly-alpha-L-guluronic acid
-
 Alteromonas sp. ?
-
 ?
poly-alpha-L-guluronic acid
-
 Alteromonas sp. unsaturated 1,4-di-, 1,4-tri- and 1,4-tetrasaccharides of L-guluronate
-
 ?
poly-beta1,4-D-mannuronan competitive to poly-alpha1,4-L-guluronan Alteromonas sp. ?
-
 ?
saturated hepta((1-4)-alpha-L-guluronan)
-
 Alteromonas sp. ?
-
 ?
saturated hexa((1-4)-alpha-L-guluronan)
-
 Alteromonas sp. unsaturated tetramer and a saturated dimer
-
 ?
saturated penta((1-4)-alpha-L-guluronan)
-
 Alteromonas sp. ?
-
 ?
saturated tetra((1-4)-alpha-L-guluronan) only reactive in a high concentration of enzyme, and for a prolonged reaction time Alteromonas sp. ?
-
 ?

Subunits

Subunits Comment Organism
? x * 33900, SDS-PAGE Alteromonas sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Alteromonas sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
 assay at Alteromonas sp.

pH Range

pH Minimum pH Maximum Comment Organism
7.5 8 maximal activity, slightly dependent on buffer composition Alteromonas sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
5 11 stable Alteromonas sp.

pI Value

Organism Comment pI Value Maximum pI Value
Alteromonas sp.
-
-
 3.8