Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | complete inhibition at 1 mM concentration, almost completely recovered activity by the addition of 2 mM CaCl2 or partially recovered by the addition of CoCl2 or MnCl2, suggesting that the enzyme conformation is bivalent-cation dependent | Bacillus sp. (in: Bacteria) | |
Guanidinium chloride | concentrations above 3 M cause significant loss of activity | Bacillus sp. (in: Bacteria) | |
additional information | 10 mM SDS, protein denaturants or 1 mM urea do not affect enzyme activity | Bacillus sp. (in: Bacteria) | |
Urea | 36% inhibition in the presence of 8 M urea | Bacillus sp. (in: Bacteria) |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | 2 mM, 66% inhibition | Bacillus sp. (in: Bacteria) |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
39000 | - |
Sephacryl S-200 HR column chromatography | Bacillus sp. (in: Bacteria) |
41000 | - |
1 * 41000, SDS-PAGE | Bacillus sp. (in: Bacteria) |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
poly-alpha-L-guluronic acid | Bacillus sp. (in: Bacteria) | - |
unsaturated 1,4-di-, 1,4-tri- and 1,4-tetrasaccharides of L-guluronate | - |
? | |
poly-alpha-L-guluronic acid | Bacillus sp. (in: Bacteria) ATB-1015 | - |
unsaturated 1,4-di-, 1,4-tri- and 1,4-tetrasaccharides of L-guluronate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus sp. (in: Bacteria) | - |
ATB-1015 strain | - |
Bacillus sp. (in: Bacteria) ATB-1015 | - |
ATB-1015 strain | - |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation, gel filtration and anion-exchange chromatography | Bacillus sp. (in: Bacteria) |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
poly-alpha-L-guluronic acid | - |
Bacillus sp. (in: Bacteria) | ? | - |
? | |
poly-alpha-L-guluronic acid | - |
Bacillus sp. (in: Bacteria) ATB-1015 | ? | - |
? | |
poly-alpha-L-guluronic acid | - |
Bacillus sp. (in: Bacteria) | unsaturated 1,4-di-, 1,4-tri- and 1,4-tetrasaccharides of L-guluronate | - |
? | |
poly-alpha-L-guluronic acid | - |
Bacillus sp. (in: Bacteria) ATB-1015 | unsaturated 1,4-di-, 1,4-tri- and 1,4-tetrasaccharides of L-guluronate | - |
? | |
poly-beta1,4-D-mannuronan | seems to have slight activity on poly-mannuronan | Bacillus sp. (in: Bacteria) | ? | - |
? | |
poly-beta1,4-D-mannuronan | seems to have slight activity on poly-mannuronan | Bacillus sp. (in: Bacteria) ATB-1015 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 41000, SDS-PAGE | Bacillus sp. (in: Bacteria) |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
- |
Bacillus sp. (in: Bacteria) |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
50% of the activity remains after treatment at this temperature for 30 min | Bacillus sp. (in: Bacteria) |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Bacillus sp. (in: Bacteria) |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7 | 8 | about 70% activity retained at pH 9.0 | Bacillus sp. (in: Bacteria) |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5 | - |
relatively stable in alkaline pH and unstable in a range outside acidic pH 5.0 | Bacillus sp. (in: Bacteria) |