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Literature summary for 4.2.2.10 extracted from

  • Xu, S.X.; Qin, X.; Liu, B.; Zhang, D.Q.; Zhang, W.; Wu, K.; Zhang, Y.H.
    An acidic pectin lyase from Aspergillus niger with favourable efficiency in fruit juice clarification (2015), Lett. Appl. Microbiol., 60, 181-187 .
    View publication on PubMed

Application

Application Comment Organism
food industry pectin lyase PNL-ZJ5A can effectively decrease the viscosity and improve the yield of apple juice and light transmittance Aspergillus niger

Cloned(Commentary)

Cloned (Comment) Organism
expression in Pichia pastoris Aspergillus niger

Inhibitors

Inhibitors Comment Organism Structure
EDTA 10 mM, more than 50% inhibition Aspergillus niger
Li+ 10 mM, 40% inhibition Aspergillus niger
Ni2+ 10 mM, more than 50% inhibition Aspergillus niger
SDS 10 mM, more than 50% inhibition Aspergillus niger
Zn2+ 10 mM, more than 50% inhibition Aspergillus niger

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
citrus pectin Km value 0.66 mg/ml, pH 5.0, 40°C Aspergillus niger

Organism

Organism UniProt Comment Textmining
Aspergillus niger
-
-
-
Aspergillus niger ZJ5
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
26.85
-
pH 5.0, 43°C Aspergillus niger

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
citrus pectin
-
Aspergillus niger ?
-
?
citrus pectin
-
Aspergillus niger ZJ5 ?
-
?
pectin with 70% methyl esterification 44% of the activity with citrus pectin Aspergillus niger ?
-
?
pectin with 70% methyl esterification 44% of the activity with citrus pectin Aspergillus niger ZJ5 ?
-
?
pectin with 85% methyl esterification 118% of the activity with citrus pectin Aspergillus niger ?
-
?
pectin with 85% methyl esterification 118% of the activity with citrus pectin Aspergillus niger ZJ5 ?
-
?

Synonyms

Synonyms Comment Organism
pnl-zj5a
-
Aspergillus niger

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
43
-
-
Aspergillus niger

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
43
-
1 h, pH 5.0, 77% residual activity Aspergillus niger

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
-
Aspergillus niger

pH Range

pH Minimum pH Maximum Comment Organism
4.3 5.5
-
Aspergillus niger

pH Stability

pH Stability pH Stability Maximum Comment Organism
3 6 1 h, 37°C, more than 70% residual activity Aspergillus niger