Application | Comment | Organism |
---|---|---|
food industry | pectin lyase PNL-ZJ5A can effectively decrease the viscosity and improve the yield of apple juice and light transmittance | Aspergillus niger |
Cloned (Comment) | Organism |
---|---|
expression in Pichia pastoris | Aspergillus niger |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | 10 mM, more than 50% inhibition | Aspergillus niger | |
Li+ | 10 mM, 40% inhibition | Aspergillus niger | |
Ni2+ | 10 mM, more than 50% inhibition | Aspergillus niger | |
SDS | 10 mM, more than 50% inhibition | Aspergillus niger | |
Zn2+ | 10 mM, more than 50% inhibition | Aspergillus niger |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
citrus pectin | Km value 0.66 mg/ml, pH 5.0, 40°C | Aspergillus niger |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus niger | - |
- |
- |
Aspergillus niger ZJ5 | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
26.85 | - |
pH 5.0, 43°C | Aspergillus niger |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
citrus pectin | - |
Aspergillus niger | ? | - |
? | |
citrus pectin | - |
Aspergillus niger ZJ5 | ? | - |
? | |
pectin with 70% methyl esterification | 44% of the activity with citrus pectin | Aspergillus niger | ? | - |
? | |
pectin with 70% methyl esterification | 44% of the activity with citrus pectin | Aspergillus niger ZJ5 | ? | - |
? | |
pectin with 85% methyl esterification | 118% of the activity with citrus pectin | Aspergillus niger | ? | - |
? | |
pectin with 85% methyl esterification | 118% of the activity with citrus pectin | Aspergillus niger ZJ5 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
pnl-zj5a | - |
Aspergillus niger |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
43 | - |
- |
Aspergillus niger |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
43 | - |
1 h, pH 5.0, 77% residual activity | Aspergillus niger |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
- |
Aspergillus niger |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4.3 | 5.5 | - |
Aspergillus niger |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
3 | 6 | 1 h, 37°C, more than 70% residual activity | Aspergillus niger |