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Literature summary for 4.2.2.10 extracted from

  • Yadav, S.; Maurya, S.; Anand, G.; Dwivedi, R.; Yadav, D.
    Purification and characterization of a highly alkaline pectin lyase from Fusarium lateritum MTCC 8794 (2017), Biologia, 72, 245-251 .
No PubMed abstract available
Search for more literature for 4.2.2.10

Activating Compound

Activating Compound Comment Organism Structure
ammonium sulfate 1 mM, 203% of initial activity Fusarium lateritium
azide 1 mM, 125% of initial activity Fusarium lateritium

Inhibitors

Inhibitors Comment Organism Structure
arsenate 1 mM, 92% of initial activity Fusarium lateritium
Ca2+ 1 mM, 2886% of initial activity, 5 mM, 44% of initial activity Fusarium lateritium
Citric acid 1 mM, 78% of initial activity Fusarium lateritium
EDTA 1 mM, 10% of initial activity Fusarium lateritium
ferrocyanide 1 mM, complete loss of activity Fusarium lateritium
Mg2+ 5 mM, 72% of initial activity Fusarium lateritium
Permanganate 1 mM, complete loss of activity Fusarium lateritium

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 citrus pectin Km value 1.75 mg/ml, pH 9.0, 37°C Fusarium lateritium

Metals/Ions

Metals/Ions Comment Organism Structure
Ag+ 1 mM, 416% of initial activity Fusarium lateritium
Ca2+ 1 mM, 2886% of initial activity, 5 mM, 44% of initial activity Fusarium lateritium
Co2+ 1 mM, 336% of initial activity Fusarium lateritium
Hg2+ 1 mM, 360% of initial activity Fusarium lateritium
K+ 1 mM, 200% of initial activity Fusarium lateritium
Na+ 1 mM, 180% of initial activity Fusarium lateritium
Zn2+ 1 mM, 130% of initial activity Fusarium lateritium

Organism

Organism UniProt Comment Textmining
Fusarium lateritium
-
-
-
Fusarium lateritium MTCC 8794
-
-
-

Purification (Commentary)

Purification (Comment) Organism
purification by ammonium sulfate fractionation, cation-exchange chromatography on carboxymethyl cellulose followed by gel filtration chromatography Fusarium lateritium

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2
-
 pH 9.0, 37°C Fusarium lateritium

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
citrus pectin
-
 Fusarium lateritium ?
-
 ?
citrus pectin
-
 Fusarium lateritium MTCC 8794 ?
-
 ?

Subunits

Subunits Comment Organism
? * 31000, SDS-PAGE Fusarium lateritium

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
 Fusarium lateritium

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
10 50 stable for 30 min Fusarium lateritium
60
-
 30 min, 32% loss of activity Fusarium lateritium

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
83.3
-
 citrus pectin pH 9.0, 37°C Fusarium lateritium

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
-
 Fusarium lateritium

pH Range

pH Minimum pH Maximum Comment Organism
9 10 more than 98% of maximum activity Fusarium lateritium

pH Stability

pH Stability pH Stability Maximum Comment Organism
5 7 stable for 24 h Fusarium lateritium

General Information

General Information Comment Organism
physiological function retting of Crotolaria juncea sticks by the purified enzyme is accomplished in the absence of EDTA Fusarium lateritium