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Literature summary for 4.2.2.10 extracted from

  • Yadav, S.; Dubey, A.K.; Anand, G.; Yadav, D.
    Characterization of a neutral pectin lyase produced by Oidiodendron echinulatum MTCC 1356 in solid state fermentation (2012), J. Basic Microbiol., 52, 713-720.
    View publication on PubMed
Search for more literature for 4.2.2.10

Activating Compound

Activating Compound Comment Organism Structure
EDTA 1 mM, 123% of initial activity Oidiodendron echinulatum

Application

Application Comment Organism
industry enzyme shows retting of Crotalaria juncea fibre in the presence of EDTA Oidiodendron echinulatum

Inhibitors

Inhibitors Comment Organism Structure
Ag+ 1 mM, complete inhibition Oidiodendron echinulatum
Cu2+ 1 mM, complete inhibition Oidiodendron echinulatum
KMnO4 1 mM, complete inhibition Oidiodendron echinulatum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 citrus pectin Km value is 1.2 mg/ml, pH 7.0, 37°C Oidiodendron echinulatum

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ 1 mM, 137% of initial activity Oidiodendron echinulatum
Mg2+ 1 mM, 137% of initial activity Oidiodendron echinulatum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
42000
-
 1 * 42000, SDS-PAGE Oidiodendron echinulatum
47000
-
 PAGE Oidiodendron echinulatum

Organism

Organism UniProt Comment Textmining
Oidiodendron echinulatum
-
-
-
Oidiodendron echinulatum MTCC 1356
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Oidiodendron echinulatum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.5
-
 pH 7.0, 37°C Oidiodendron echinulatum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
citrus pectin
-
 Oidiodendron echinulatum ?
-
 ?
citrus pectin
-
 Oidiodendron echinulatum MTCC 1356 ?
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 42000, SDS-PAGE Oidiodendron echinulatum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
 Oidiodendron echinulatum

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 stable for at least 30 min Oidiodendron echinulatum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
 Oidiodendron echinulatum