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Literature summary for 4.2.2.10 extracted from

  • Nikaidou, N.; Naganuma, T.; Kamio, Y.; Izaki, K.
    Production, purification, and properties of a pectin lyase from Pseudomonas marginalis N6301 (1995), Biosci. Biotechnol. Biochem., 59, 323-324.
No PubMed abstract available
Search for more literature for 4.2.2.10

Inhibitors

Inhibitors Comment Organism Structure
malic anhydride 2 mM, complete inhibition Pseudomonas marginalis
N-Chlorosuccinimide 2 mM, 21% inhibition Pseudomonas marginalis
NEM 2 mM, 96% inhibition Pseudomonas marginalis
p-chloromercuriphenylsulfonic acid 2 mM, complete inhibition Pseudomonas marginalis
Phenylmethylsulfonylfluoride 2 mM, 9% inhibition Pseudomonas marginalis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
34000
-
 x * 34000, SDS-PAGE Pseudomonas marginalis

Organism

Organism UniProt Comment Textmining
Pseudomonas marginalis
-
 N-6031
-
Pseudomonas marginalis N-6031
-
 N-6031
-

Purification (Commentary)

Purification (Comment) Organism
-
 Pseudomonas marginalis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
pectin
-
 Pseudomonas marginalis ?
-
 ?
pectin
-
 Pseudomonas marginalis N-6031 ?
-
 ?

Subunits

Subunits Comment Organism
? x * 34000, SDS-PAGE Pseudomonas marginalis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
 pH 8.5, 30 min, stable below Pseudomonas marginalis
45
-
 rapid loss of activity Pseudomonas marginalis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Pseudomonas marginalis

pH Stability

pH Stability pH Stability Maximum Comment Organism
5.2 8.1 stable Pseudomonas marginalis