Cloned (Comment) | Organism |
---|---|
the 18 amino acid-truncated mutant E87T cannot be overexpressed in Escherichia coli | Homo sapiens |
wild type and mutant enzyme C82R overexpressed in Escherichia coli | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
C82R | mutant enzyme C82R reveals 60% decrease in Vmax and a slight decrease in Km-value for 4a-hydroxytetrahydrobiopterin. The susceptibility to proteolysis of mutant C82R, however is markedly increased compared with the wild type enzyme | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0047 | - |
4a-hydroxytetrahydrobiopterin | mutant enzyme C82R | Homo sapiens | |
0.0052 | - |
4a-hydroxytetrahydrobiopterin | wild type enzyme | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Homo sapiens | the mutant enzyme form C82R and the 18-amino acid-truncated mutant Glu87-termination occur naturally associated with hyperphenyalaninemia | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4a-hydroxytetrahydrobiopterin | - |
Homo sapiens | ? | - |
? | |
additional information | the mutant enzyme form C82R and the 18-amino acid-truncated mutant Glu87-termination occur naturally associated with hyperphenyalaninemia | Homo sapiens | ? | - |
? |