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Literature summary for 4.2.1.96 extracted from

  • Johen, G.; Kowlessur, D.; Citron, B.A.; Kaufman, S.
    Characterization of the wild-type form of 4a-carbinolamine dehydratase and two naturally occuring mutants associated with hyperphenylalaninemia (1995), Proc. Natl. Acad. Sci. USA, 92, 12384-12388.
    View publication on PubMed
Search for more literature for 4.2.1.96

Cloned(Commentary)

Cloned (Comment) Organism
the 18 amino acid-truncated mutant E87T cannot be overexpressed in Escherichia coli Homo sapiens
wild type and mutant enzyme C82R overexpressed in Escherichia coli Homo sapiens

Protein Variants

Protein Variants Comment Organism
C82R mutant enzyme C82R reveals 60% decrease in Vmax and a slight decrease in Km-value for 4a-hydroxytetrahydrobiopterin. The susceptibility to proteolysis of mutant C82R, however is markedly increased compared with the wild type enzyme Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0047
-
 4a-hydroxytetrahydrobiopterin mutant enzyme C82R Homo sapiens
0.0052
-
 4a-hydroxytetrahydrobiopterin wild type enzyme Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Homo sapiens the mutant enzyme form C82R and the 18-amino acid-truncated mutant Glu87-termination occur naturally associated with hyperphenyalaninemia ?
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4a-hydroxytetrahydrobiopterin
-
 Homo sapiens ?
-
 ?
additional information the mutant enzyme form C82R and the 18-amino acid-truncated mutant Glu87-termination occur naturally associated with hyperphenyalaninemia Homo sapiens ?
-
 ?