Cloned (Comment) | Organism |
---|---|
wild-type and mutant enzymes | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
E57A | the His79Ala mutant and the His79Ser mutant exhibit about 40% the activity of the wild-type enzyme. In the mutant enzymes His61Ala and His62Ala the activity is reduced to 10%. In the mutant enzymes Asp60Ala and Arg87Ala the activity is reduced to 30%. The Glu57Ala mutant and the His61Ala,His62Ala double mutant show no activity | Homo sapiens |
H61A | the His79Ala mutant and the His79Ser mutant exhibit about 40% the activity of the wild-type enzyme. In the mutant enzymes His61Ala and His62Ala the activity is reduced to 10%. In the mutant enzymes Asp60Ala and Arg87Ala the activity is reduced to 30%. The Glu57Ala mutant and the His61Ala,His62Ala double mutant show no activity | Homo sapiens |
H61A/H62A | the His79Ala mutant and the His79Ser mutant exhibit about 40% the activity of the wild-type enzyme. In the mutant enzymes His61Ala and His62Ala the activity is reduced to 10%. In the mutant enzymes Asp60Ala and Arg87Ala the activity is reduced to 30%. The Glu57Ala mutant and the His61Ala,His62Ala double mutant show no activity | Homo sapiens |
H62A | the His79Ala mutant and the His79Ser mutant exhibit about 40% the activity of the wild-type enzyme. In the mutant enzymes His61Ala and His62Ala the activity is reduced to 10%. In the mutant enzymes Asp60Ala and Arg87Ala the activity is reduced to 30%. The Glu57Ala mutant and the His61Ala,His62Ala double mutant show no activity | Homo sapiens |
H79A | the His79Ala mutant and the His79Ser mutant exhibit about 40% the activity of the wild-type enzyme. In the mutant enzymes His61Ala and His62Ala the activity is reduced to 10%. In the mutant enzymes Asp60Ala and Arg87Ala the activity is reduced to 30%. The Glu57Ala mutant and the His61Ala,His62Ala double mutant show no activity | Homo sapiens |
H79S | the His79Ala mutant and the His79Ser mutant exhibit about 40% the activity of the wild-type enzyme. In the mutant enzymes His61Ala and His62Ala the activity is reduced to 10%. In the mutant enzymes Asp60Ala and Arg87Ala the activity is reduced to 30%. The Glu57Ala mutant and the His61Ala,His62Ala double mutant show no activity | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.007 | - |
6(S)-methyl-7,8-dihydropterin-4a-carbinolamine | mutant enzyme Tyr69Thr | Homo sapiens | |
0.008 | - |
6(S)-methyl-7,8-dihydropterin-4a-carbinolamine | mutant enzyme Y69F | Homo sapiens | |
0.01 | - |
6(S)-methyl-7,8-dihydropterin-4a-carbinolamine | mutant enzyme H79A | Homo sapiens | |
0.012 | - |
6(S)-methyl-7,8-dihydropterin-4a-carbinolamine | mutant enzyme His79Ser | Homo sapiens | |
0.017 | - |
6(S)-methyl-7,8-dihydropterin-4a-carbinolamine | mutant enzyme Cys81Arg | Homo sapiens | |
0.018 | - |
6(S)-methyl-7,8-dihydropterin-4a-carbinolamine | mutant enzyme D60A | Homo sapiens | |
0.02 | - |
6(S)-methyl-7,8-dihydropterin-4a-carbinolamine | mutant enzyme Arg87Ala | Homo sapiens | |
0.025 | - |
6(S)-methyl-7,8-dihydropterin-4a-carbinolamine | wild type enzyme | Homo sapiens | |
0.04 | - |
6(S)-methyl-7,8-dihydropterin-4a-carbinolamine | mutant enzyme Cys81Ser | Homo sapiens | |
0.075 | - |
6(S)-methyl-7,8-dihydropterin-4a-carbinolamine | mutant enzyme H62A | Homo sapiens | |
2.2 | - |
6(S)-methyl-7,8-dihydropterin-4a-carbinolamine | mutant enzyme H61A | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Rattus norvegicus | - |
- |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
4a-hydroxytetrahydrobiopterin = 6,7-dihydrobiopterin + H2O | the mechanism of dehydration involves base catalysis at the N(5)-H group of the substrate by His61 | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Homo sapiens | - |
liver | - |
Rattus norvegicus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
6(S)-methyl-7,8-dihydropterin-4a-carbinolamine | - |
Homo sapiens | ? | - |
? |