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Literature summary for 4.2.1.96 extracted from

  • K๖ster, S.; Stier, G.; Kubasch, N.; Curtius, H.C.; Ghisla, S.
    Pterin-4alpha-carbinolamine dehydratase from Pseudomonas aeruginosa: characterization, catalytic mechanism and comparison to the human enzyme (1998), Biol. Chem., 379, 1427-1432.
    View publication on PubMed
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Protein Variants

Protein Variants Comment Organism
D100N the single mutants H73A and H74A and the double mutant H73A,H74A are completely inactive. The activity of the mutant enzymes H91A and E69A is 40% of the activity of the wild type enzyme. The activity of the mutant enzymes D100N is 10% of the activity of the wild type enzyme Pseudomonas aeruginosa
E69A the single mutants H73A and H74A and the double mutant H73A,H74A are completely inactive. The activity of the mutant enzymes H91A and E69A is 40% of the activity of the wild type enzyme. The activity of the mutant enzymes D100N is 10% of the activity of the wild type enzyme Pseudomonas aeruginosa
H73A the single mutants H73A and H74A and the double mutant H73A,H74A are completely inactive. The activity of the mutant enzymes H91A and E69A is 40% of the activity of the wild type enzyme. The activity of the mutant enzymes D100N is 10% of the activity of the wild type enzyme Pseudomonas aeruginosa
H73A/H74A the single mutants H73A and H74A and the double mutant H73A,H74A are completely inactive. The activity of the mutant enzymes H91A and E69A is 40% of the activity of the wild type enzyme. The activity of the mutant enzymes D100N is 10% of the activity of the wild type enzyme Pseudomonas aeruginosa
H74A the single mutants H73A and H74A and the double mutant H73A,H74A are completely inactive. The activity of the mutant enzymes H91A and E69A is 40% of the activity of the wild type enzyme. The activity of the mutant enzymes D100N is 10% of the activity of the wild type enzyme Pseudomonas aeruginosa
H91A the single mutants H73A and H74A and the double mutant H73A,H74A are completely inactive. The activity of the mutant enzymes H91A and E69A is 40% of the activity of the wild type enzyme. The activity of the mutant enzymes D100N is 10% of the activity of the wild type enzyme Pseudomonas aeruginosa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.008
-
 6(S)-methyl-7,8-dihydropterin-4a-carbinolamine mutant enzyme Y69F Homo sapiens
0.01
-
 6(S)-methyl-7,8-dihydropterin-4a-carbinolamine mutant enzyme H79A Homo sapiens
0.018
-
 6(S)-methyl-7,8-dihydropterin-4a-carbinolamine mutant enzyme D60A Homo sapiens
0.02
-
 6(S)-methyl-7,8-dihydropterin-4a-carbinolamine
-
 Homo sapiens
0.02
-
 6(S)-methyl-7,8-dihydropterin-4a-carbinolamine wild type enzyme and mutant enzyme G72D Pseudomonas aeruginosa
0.025
-
 6(S)-methyl-7,8-dihydropterin-4a-carbinolamine mutant enzyme E69A Pseudomonas aeruginosa
0.025
-
 6(S)-methyl-7,8-dihydropterin-4a-carbinolamine wild type enzyme Homo sapiens
0.06
-
 6(S)-methyl-7,8-dihydropterin-4a-carbinolamine mutant enzyme H91A Pseudomonas aeruginosa
0.075
-
 6(S)-methyl-7,8-dihydropterin-4a-carbinolamine mutant enzyme H62A Homo sapiens
2.2
-
 6(S)-methyl-7,8-dihydropterin-4a-carbinolamine mutant enzyme H61A Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
 wild type and mutant enzymes E57A, H61A, H62A, H79A, Y69F, and D60A and double-mutant H61A,H62A expressed in Escherichia coli
-
Pseudomonas aeruginosa
-
 wild type and mutant enzymes E69A, D100N, H73A, H74A, H73A,H74A, H91A, W81A, and G72S expressed in Escherichia coli
-

Purification (Commentary)

Purification (Comment) Organism
wild type and mutant enzymes E69A, D100N, H73A, H74A, H91A, W81A, and G72S and double-mutant H73A,H74A expressed in Escherichia coli Pseudomonas aeruginosa

Reaction

Reaction Comment Organism Reaction ID
4a-hydroxytetrahydrobiopterin = 6,7-dihydrobiopterin + H2O mechanism Pseudomonas aeruginosa
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4a-hydroxy-tetrahydropterin
-
 Homo sapiens 7,8-dihydropterin + H2O
-
 ?
4a-hydroxy-tetrahydropterin
-
 Pseudomonas aeruginosa 7,8-dihydropterin + H2O
-
 ?
6(S)-methyl-4a-hydroxy-tetrahydropterin
-
 Pseudomonas aeruginosa 6(S)-methyl-7,8-dihydropterin + H2O
-
 ?
6(S)-methyl-7,8-dihydropterin-4a-carbinolamine
-
 Pseudomonas aeruginosa ?
-
 ?
6(S)-methyl-7,8-dihydropterin-4a-carbinolamine
-
 Homo sapiens ?
-
 ?