Activating Compound | Comment | Organism | Structure |
---|---|---|---|
glycine | best at 2.5% w/v, 28.7% activation | Olea europaea | |
additional information | for enzyme stability glycine (2.5% w/v), NaCl (0.5 M), and Na2SO4 (0.25 M) provide the highest activation of HPL full-length activity, while the best matured HPL activity is obtained with Na2SO4 (0.25 M) and NaCl (1 M), while for enzyme activity and C6-aldehyde production, glycine, NaCl, and Na2SO4 are appropriate additives and NaCl appears to be the best additive, at least for hexanal production | Olea europaea | |
Na2SO4 | best at 0.25 M | Olea europaea | |
NaCl | best at 0.5 M | Olea europaea |
Application | Comment | Organism |
---|---|---|
synthesis | the recombinant 13-HPL enzyme is useful biocatalyst to produce C6-aldehydes | Olea europaea |
Cloned (Comment) | Organism |
---|---|
recombinant expression of His-tagged full-length enzyme or matured enzyme with truncated chloroplast transit peptide in Escherichia coli strain M15 | Olea europaea |
Protein Variants | Comment | Organism |
---|---|---|
additional information | optimization of recombinant full-length and truncated enzymes stabilization by additives for production of C6-aldehydes, overview | Olea europaea |
General Stability | Organism |
---|---|
for enzyme stability glycine (2.5% w/v), NaCl (0.5 M), and Na2SO4 (0.25 M) provide the highest activation of HPL full-length activity, while the best matured HPL activity is obtained with Na2SO4 (0.25 M) and NaCl (1 M), while for enzyme activity and C6-aldehyde production, glycine, NaCl, and Na2SO4 are appropriate additives and NaCl appears to be the best additive, at least for hexanal production | Olea europaea |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(9Z,11E,13S)-13-hydroperoxy-9,11-octadecadienoic acid | Olea europaea | - |
hexanal + 12-oxo-9-dodecenoic acid | - |
? | |
(9Z,11E,13S,15Z)-13-hydroperoxy-9,11,15-octadecatrienoic acid | Olea europaea | - |
(Z)-3-hexenal + 12-oxo-9-dodecenoic acid | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Olea europaea | G8XQN2 | Leccino variety | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
proteolytic modification | the matured enzyme lacks the chloroplast transit peptide, which is cleaved off for maturation | Olea europaea |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged full-length and truncated enzymes from Escherichia coli strain M15 by cobalt affinity chromatography | Olea europaea |
Storage Stability | Organism |
---|---|
-20°C--80°C, purified recombinant full-length and matured enzymes, 100% of the rHPL activities are maintained during 5 weeks of storage in the presence of 10% v/v glycerol (best), about 70% activity are remaining at 40% glycerol after 5 weeks. Freezing without a cryoprotectant inactivates both enzymes rapidly. The enzyme activity is greatly reduced within 3 weeks for HPL full-length and within 1 week for matured HPL since only about 20% of the enzyme activity remain when they are stored at -20°C or -80°C | Olea europaea |
4°C, purified recombinant full-length and matured enzymes, quite stable for 5 weeks | Olea europaea |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(9Z,11E,13S)-13-hydroperoxy-9,11-octadecadienoic acid | - |
Olea europaea | hexanal + 12-oxo-9-dodecenoic acid | - |
? | |
(9Z,11E,13S,15Z)-13-hydroperoxy-9,11,15-octadecatrienoic acid | - |
Olea europaea | (Z)-3-hexenal + 12-oxo-9-dodecenoic acid | - |
? |
Synonyms | Comment | Organism |
---|---|---|
13-HPL | - |
Olea europaea |
13-hydroperoxide lyase | - |
Olea europaea |
HPL | - |
Olea europaea |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
- |
Olea europaea |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
17.89 | - |
(9Z,11E,13S)-13-hydroperoxy-9,11-octadecadienoic acid | pH 7.5, 25°C, recombinant truncated enzyme | Olea europaea | |
26.38 | - |
(9Z,11E,13S)-13-hydroperoxy-9,11-octadecadienoic acid | pH 7.5, 25°C, recombinant full-length enzyme | Olea europaea | |
42 | - |
(9Z,11E,13S)-13-hydroperoxy-9,11-octadecadienoic acid | pH 7.5, 25°C, recombinant full-length enzyme, with 2.5% glycine w/v | Olea europaea | |
46.9 | - |
(9Z,11E,13S)-13-hydroperoxy-9,11-octadecadienoic acid | pH 7.5, 25°C, recombinant truncated enzyme, with 2.5% glycine w/v | Olea europaea | |
73.2 | - |
(9Z,11E,13S)-13-hydroperoxy-9,11-octadecadienoic acid | pH 7.5, 25°C, recombinant truncated enzyme, with 0.5 M NaCl | Olea europaea | |
133.9 | - |
(9Z,11E,13S)-13-hydroperoxy-9,11-octadecadienoic acid | pH 7.5, 25°C, recombinant full-length enzyme, with 0.5 M NaCl | Olea europaea | |
402.62 | - |
(9Z,11E,13S,15Z)-13-hydroperoxy-9,11,15-octadecatrienoic acid | pH 7.5, 25°C, recombinant truncated enzyme | Olea europaea | |
564.95 | - |
(9Z,11E,13S,15Z)-13-hydroperoxy-9,11,15-octadecatrienoic acid | pH 7.5, 25°C, recombinant full-length enzyme | Olea europaea | |
579 | - |
(9Z,11E,13S,15Z)-13-hydroperoxy-9,11,15-octadecatrienoic acid | pH 7.5, 25°C, recombinant full-length enzyme, with 0.5 M NaCl | Olea europaea | |
587.4 | - |
(9Z,11E,13S,15Z)-13-hydroperoxy-9,11,15-octadecatrienoic acid | pH 7.5, 25°C, recombinant full-length enzyme, with 2.5% glycine w/v | Olea europaea | |
701.6 | - |
(9Z,11E,13S,15Z)-13-hydroperoxy-9,11,15-octadecatrienoic acid | pH 7.5, 25°C, recombinant truncated enzyme, with 0.5 M NaCl | Olea europaea | |
843.3 | - |
(9Z,11E,13S,15Z)-13-hydroperoxy-9,11,15-octadecatrienoic acid | pH 7.5, 25°C, recombinant truncated enzyme, with 2.5% glycine w/v | Olea europaea |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Olea europaea |
General Information | Comment | Organism |
---|---|---|
metabolism | hydroperoxide lyase (HPL) is an enzyme found in higher plants that is involved in a lipid oxidation pathway, called the lipoxygenase pathway, which is activated in response to wounds or pathogen attacks | Olea europaea |
physiological function | hydroperoxide lyase (HPL) is an enzyme found in higher plants that is involved in a lipid oxidation pathway, called the lipoxygenase pathway, which is activated in response to wounds or pathogen attacks. Volatile C6-aldehydes and C9-aldehydes produced by HPLs and their corresponding alcohols are known as green leaf volatiles (GLVs) and are responsible for the fresh green odor of many fruits and vegetables. GLVs such as hexanal, (Z)-3-hexenal, and (E)-2-hexenal are associated to the green note odor that is of major importance in the fragrance and flavor industries. They are also widely used in the food and beverage industry to restore the fresh green character lost during processing or to enhance food storage due to their antimicrobial activities | Olea europaea |