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Literature summary for 4.2.1.91 extracted from

  • Maeda, H.; Shasany, A.K.; Schnepp, J.; Orlova, I.; Taguchi, G.; Cooper, B.R.; Rhodes, D.; Pichersky, E.; Dudareva, N.
    RNAi suppression of arogenate dehydratase1 reveals that phenylalanine is synthesized predominantly via the arogenate pathway in petunia petals (2010), Plant Cell, 22, 832-849.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
coding regions corresponding to mature ADT protein (lacking the N-terminal plastid transit peptides) subcloned into the expression vector pET-28a, which contains an N-terminal 6xHis tag. Recombinant protein expressed in Escherichia coli Rosetta cells Petunia x hybrida

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0488
-
L-arogenate at 37°C, in 250 mM Na-phosphate buffer, pH 8.2, 1 mM EDTA Petunia x hybrida
0.0667
-
L-arogenate at 37°C, in 250 mM Na-phosphate buffer, pH 8.2, 1 mM EDTA Petunia x hybrida
0.179
-
L-arogenate at 37°C, in 250 mM Na-phosphate buffer, pH 8.2, 1 mM EDTA Petunia x hybrida
0.4658
-
prephenate at 37°C, in 250 mM Na-phosphate buffer, pH 8.2, 1 mM EDTA Petunia x hybrida
0.752
-
prephenate at 37°C, in 250 mM Na-phosphate buffer, pH 8.2, 1 mM EDTA Petunia x hybrida

Localization

Localization Comment Organism GeneOntology No. Textmining
chloroplast
-
Petunia x hybrida 9507
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
43400
-
sequence analysis Petunia x hybrida
45900
-
sequence analysis Petunia x hybrida
47300
-
sequence analysis Petunia x hybrida

Organism

Organism UniProt Comment Textmining
Petunia x hybrida D3U715
-
-
Petunia x hybrida D3U716
-
-
Petunia x hybrida D3U717
-
-

Purification (Commentary)

Purification (Comment) Organism
by fast protein liquid chromatography and gel filtration Petunia x hybrida

Source Tissue

Source Tissue Comment Organism Textmining
flower
-
Petunia x hybrida
-
leaf
-
Petunia x hybrida
-
petal
-
Petunia x hybrida
-
stem only ADT1 and ADT3 Petunia x hybrida
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-arogenate
-
Petunia x hybrida L-phenylalanine + CO2 + H2O
-
?
L-arogenate ADT1 shows strict substrate specificity toward arogenate, although with the lowest catalytic efficiency among the three ADTs Petunia x hybrida L-phenylalanine + CO2 + H2O
-
?
prephenate
-
Petunia x hybrida phenylpyruvate + CO2 + H2O
-
?

Synonyms

Synonyms Comment Organism
ADT1
-
Petunia x hybrida
ADT2
-
Petunia x hybrida
ADT3
-
Petunia x hybrida
arogenate dehydratase1
-
Petunia x hybrida
arogenate dehydratase2
-
Petunia x hybrida
arogenate dehydratase3
-
Petunia x hybrida

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.012
-
prephenate at 37°C, in 250 mM Na-phosphate buffer, pH 8.2, 1 mM EDTA Petunia x hybrida
0.06
-
prephenate at 37°C, in 250 mM Na-phosphate buffer, pH 8.2, 1 mM EDTA Petunia x hybrida
0.194
-
L-arogenate at 37°C, in 250 mM Na-phosphate buffer, pH 8.2, 1 mM EDTA Petunia x hybrida
0.267
-
L-arogenate at 37°C, in 250 mM Na-phosphate buffer, pH 8.2, 1 mM EDTA Petunia x hybrida
1.231
-
L-arogenate at 37°C, in 250 mM Na-phosphate buffer, pH 8.2, 1 mM EDTA Petunia x hybrida

Expression

Organism Comment Expression
Petunia x hybrida ADT1 suppression via RNA interference in petunia petals significantly reduces ADT activity, levels of Phe, and downstream phenylpropanoid/benzenoid volatiles, whereby arogenate levels are unaltered, while shikimate and Trp levels are decreased in transgenic petals down
Petunia x hybrida ADT1 exhibits the highest level of expression in corolla and tube, the scentproducing parts of petunia flowers. In corolla, the ADT1 transcript levels exceed those of ADT2 and ADT3 by 88- and 10fold, respectively. ADT1 expression is positively correlated with endogenous Phe levels throughout flower development up
Petunia x hybrida in corolla, the ADT1 transcript levels exceed those of ADT2 and ADT3 by 88- and 10fold, respectively up

General Information

General Information Comment Organism
malfunction ADT1 suppression leads to downregulation of carbon flux toward shikimic acid, but exogenous supply of shikimate bypasses this negative regulation and results in elevated arogenate accumulation Petunia x hybrida

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.03
-
prephenate at 37°C, in 250 mM Na-phosphate buffer, pH 8.2, 1 mM EDTA Petunia x hybrida
0.08
-
prephenate at 37°C, in 250 mM Na-phosphate buffer, pH 8.2, 1 mM EDTA Petunia x hybrida
1.48
-
L-arogenate at 37°C, in 250 mM Na-phosphate buffer, pH 8.2, 1 mM EDTA Petunia x hybrida
4
-
L-arogenate at 37°C, in 250 mM Na-phosphate buffer, pH 8.2, 1 mM EDTA Petunia x hybrida
18.51
-
L-arogenate at 37°C, in 250 mM Na-phosphate buffer, pH 8.2, 1 mM EDTA Petunia x hybrida