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Literature summary for 4.2.1.91 extracted from
- Enzymatic and nonenzymatic dehydration reactions of L-arogenate (1985), Biochemistry, 24, 1607-1612.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| beta-2-Thienylalanine | - |
Brevundimonas diminuta |  |
| L-phenylalanine | - |
Brevundimonas diminuta | |
| additional information | not feedback inhibited by L-phenylalanine | Pseudomonas aeruginosa | |
| p-Fluorophenylalanine | - |
Brevundimonas diminuta | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.63 | - |
L-arogenate | - |
Brevundimonas diminuta | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-arogenate | Pseudomonas aeruginosa | - |
L-phenylalanine + CO2 + H2O | - |
? | |
| L-arogenate | Brevundimonas diminuta | - |
L-phenylalanine + CO2 + H2O | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Brevundimonas diminuta | - |
ATCC 13184 and ATCC 11568 | - |
| Pseudomonas aeruginosa | - |
ATCC 15692 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partially | Brevundimonas diminuta |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.0012 | - |
crude extract | Brevundimonas diminuta |
| 0.0063 | - |
ATCC 13184 | Brevundimonas diminuta |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -80°C, enzyme activity in crude extract is stable for at least 1 month | Brevundimonas diminuta |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-arogenate | - |
Pseudomonas aeruginosa | L-phenylalanine + CO2 + H2O | - |
? | |
| L-arogenate | - |
Brevundimonas diminuta | L-phenylalanine + CO2 + H2O | - |
? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 8.5 | ATCC 13184 | Brevundimonas diminuta |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 8.2 | - |
ATCC 13184, activity declines sharply above | Brevundimonas diminuta |
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