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Literature summary for 4.2.1.9 extracted from

  • Flint, D.H.; Emptage, M.H.
    Dihydroxy acid dehydratase from spinach contains a [2Fe-2S] cluster (1988), J. Biol. Chem., 263, 3558-3564.
    View publication on PubMed

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.5
-
2,3-dihydroxyisovalerate
-
Spinacia oleracea

Metals/Ions

Metals/Ions Comment Organism Structure
Iron contains a catalytically active [2Fe-2S] cluster Spinacia oleracea

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
63000
-
2 * 63000, SDS-PAGE Spinacia oleracea
105000
-
gel filtration Spinacia oleracea
110000
-
native PAGE Spinacia oleracea

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Spinacia oleracea third enzyme in the branched-chain amino acid biosynthetic pathway ?
-
?

Organism

Organism UniProt Comment Textmining
Spinacia oleracea
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Spinacia oleracea

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
Spinacia oleracea
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Spinacia oleracea

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,3-dihydroxy-3-methylbutanoate
-
Spinacia oleracea 2-oxoisovalerate + H2O
-
?
2,3-dihydroxy-3-methylpentanoate
-
Spinacia oleracea 3-methyl-2-oxopentanoate + H2O
-
?
additional information third enzyme in the branched-chain amino acid biosynthetic pathway Spinacia oleracea ?
-
?

Subunits

Subunits Comment Organism
dimer 2 * 63000, SDS-PAGE Spinacia oleracea

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
25
-
2,3-dihydroxyisovalerate
-
Spinacia oleracea