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Literature summary for 4.2.1.85 extracted from

  • Kollmann-Koch, A.; Eggerer, H.
    Nicotinic acid metabolism. Dimethylmaleate hydratase (1984), Hoppe-Seyler's Z. Physiol. Chem., 365, 847-857.
    View publication on PubMed

General Stability

General Stability Organism
unstable during purification, probably because of dissociation into inactive protein compounds Eubacterium barkeri

Inhibitors

Inhibitors Comment Organism Structure
5,5'-dithiobis(2-nitrobenzoate) 1 mM: 97% inhibition Eubacterium barkeri
EDTA 1-5 mM: 70% loss of activity Eubacterium barkeri
additional information 10 min, 25°C, 2 mM iodoacetamide or iodoacetate: no influence Eubacterium barkeri
N-ethylmaleimide 1 mM: 93% inhibition Eubacterium barkeri

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1
-
dimethylmaleate 35°C, pH 7 Eubacterium barkeri

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ required Eubacterium barkeri
additional information Fe2+ not replaceable by Ca2+, Mg2+, Mn2+ or Zn2+ Eubacterium barkeri

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
153000
-
gel filtration Eubacterium barkeri

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
dimethylmaleate + H2O Eubacterium barkeri
-
(2R,3S)-2,3-dimethylmalate
-
r

Organism

Organism UniProt Comment Textmining
Eubacterium barkeri
-
-
-

Oxidation Stability

Oxidation Stability Organism
oxygen-sensitive Eubacterium barkeri

Purification (Commentary)

Purification (Comment) Organism
ATCC 25849, partial Eubacterium barkeri

Renatured (Commentary)

Renatured (Comment) Organism
nearly completetly inactivated enzyme may be reactivated by addition of FeSO4 and glutathione, providing reducing conditions Eubacterium barkeri

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.14
-
-
Eubacterium barkeri

Storage Stability

Storage Stability Organism
-20°C, 45% loss of activity within 1 day Eubacterium barkeri
4°C, 85% loss of activity within 1 day Eubacterium barkeri
liquid N2, enzyme extract as well as crude extract stable for several weeks by addition of 20 mM citraconate and 4 mM dithiothreitol, 20 mM potassium phosphate, pH 7.0 Eubacterium barkeri

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dimethylmaleate + H2O
-
Eubacterium barkeri (2R,3S)-2,3-dimethylmalate
-
r
dimethylmaleate + H2O equilibrium 70% in favour of 2,3-dimethylmalate, dimethylfumarate is no substrate Eubacterium barkeri (2R,3S)-2,3-dimethylmalate
-
r

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
35
-
assay at Eubacterium barkeri

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5 9 broad Eubacterium barkeri