Literature summary for 4.2.1.84 extracted from

Martinez, S.; Yang, X.; Bennett, B.; Holz, R.C.
A cobalt-containing eukaryotic nitrile hydratase (2017), Biochim. Biophys. Acta, 1865, 107-112 .

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Cloned(Commentary)

Cloned (Comment)	Organism
heterologously expressed in Escherichia coli. The resulting enzyme expressed as a single polypeptide with fused alpha- and beta-subunits linked by a seventeen-histidine region. The enzyme contains its full complement of Co(III) and is fully functional without the coexpression of an activator protein or Escherichia coli GroES/EL molecular chaperones	Monosiga brevicollis

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
18	-	Benzonitrile	pH 7.0, 25°C	Monosiga brevicollis
78	-	acrylonitrile	pH 7.0, 25°C	Monosiga brevicollis

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Co2+	the enzyme expressed in the presence of 0.25 mM CoCl2 contains about 1.8 equivalents of cobalt per homodimer while the enzyme expressed in the absence of CoCl2 contains 1.6 cobalt ions per homodimer	Monosiga brevicollis

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
55000	-	SDS-PAGE	Monosiga brevicollis

Organism

Organism	UniProt	Comment	Textmining
Monosiga brevicollis	A9V2C1	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Monosiga brevicollis

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
acrylonitrile + H2O	-	Monosiga brevicollis	acrylamide	-	?
benzonitrile + H2O	-	Monosiga brevicollis	benzamide	-	?

Subunits

Subunits	Comment	Organism
homodimer	2 * 55000, SDS-PAGE	Monosiga brevicollis

Synonyms

Synonyms	Comment	Organism
MbNHase	-	Monosiga brevicollis

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
33	-	Benzonitrile	pH 7.0, 25°C	Monosiga brevicollis
130	-	acrylonitrile	pH 7.0, 25°C	Monosiga brevicollis

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
1.7	-	acrylonitrile	pH 7.0, 25°C	Monosiga brevicollis
1.8	-	Benzonitrile	pH 7.0, 25°C	Monosiga brevicollis

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Release 2023.1 (January 2023)