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Literature summary for 4.2.1.84 extracted from
- Self-subunit swapping chaperone needed for the maturation of multimeric metalloenzyme nitrile hydratase by a subunit exchange mechanism also carries out the oxidation of the metal ligand cysteine residues and insertion of cobalt (2009), J. Biol. Chem., 284, 14930-14938.
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co3+ | the incorporation of cobalt into L-NHase is depend on the alpha-subunit exchange between apo-L-NHase and NhlAE, cobalt is inserted into both the cobaltfree maturation mediator NhlAE (apo-NhlAE) and the cobalt-free alpha-subunit in an NhlE-dependent manner in the presence of cobalt and dithiothreitol in vitro | Rhodococcus fascians |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rhodococcus fascians | - |
strain DSM43985 | - |
| Rhodococcus fascians DSM 43985 | - |
strain DSM43985 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Resource Q column chromatography and Superdex-200 gel filtration | Rhodococcus fascians |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3-cyanopyridine + H2O | - |
Rhodococcus fascians | pyridine-3-carbamide | - |
? | |
| 3-cyanopyridine + H2O | - |
Rhodococcus fascians DSM 43985 | pyridine-3-carbamide | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| L-Nhase | - |
Rhodococcus fascians |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| pyrroloquinoline quinone | - |
Rhodococcus fascians | |
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Release 2023.1 (January 2023)
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