Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Rhodococcus ruber |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of an amidase-negative, amiE-, recombinant strain TH3 with 25% increased nitrile hydratase activity and 60% reduced amidase activity compared to the wild-type strain TH. Usage of TH3 free cells as biocatalysts at 18°C for acrylamide production, increased by 23% and 87% reduced acrylic acid by-product formation conmpared to the wild-type | Rhodococcus ruber |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
acrylonitrile + H2O | Rhodococcus ruber | - |
acrylamide | - |
? | |
acrylonitrile + H2O | Rhodococcus ruber TH | - |
acrylamide | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rhodococcus ruber | - |
- |
- |
Rhodococcus ruber TH | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acrylonitrile + H2O | - |
Rhodococcus ruber | acrylamide | - |
? | |
acrylonitrile + H2O | - |
Rhodococcus ruber TH | acrylamide | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
28 | - |
assay at, in vivo in cells | Rhodococcus ruber |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at, in vivo in cells | Rhodococcus ruber |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyrroloquinoline quinone | - |
Rhodococcus ruber |