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Literature summary for 4.2.1.84 extracted from

  • Rzeznicka, K.; Schaetzle, S.; Boettcher, D.; Klein, J.; Bornscheuer, U.T.
    Cloning and functional expression of a nitrile hydratase (NHase) from Rhodococcus equi TG328-2 in Escherichia coli, its purification and biochemical characterisation (2010), Appl. Microbiol. Biotechnol., 85, 1417-1425.
    View publication on PubMed
Search for more literature for 4.2.1.84

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Rhodococcus equi

Metals/Ions

Metals/Ions Comment Organism Structure
Fe3+ a Fe-type NHase Rhodococcus equi

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
23000
-
 1 * 24000 + 1 * 23000, SDS-PAGE Rhodococcus equi
24000
-
 1 * 24000 + 1 * 23000, SDS-PAGE Rhodococcus equi

Organism

Organism UniProt Comment Textmining
Rhodococcus equi
-
-
-
Rhodococcus equi TG328-2
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Sepharose 6 column chromatography Rhodococcus equi

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
844
-
 crude cell extract towards methacrylonitrile, in 50 mM Tris-HCl (pH 7.5) Rhodococcus equi
6290
-
 after 7.5fold purification towards methacrylonitrile, in 50 mM Tris-HCl (pH 7.5) Rhodococcus equi

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(R,S)-2-(4-nitrophenyl)-propionitrile + H2O 39% conversion Rhodococcus equi ?
-
 ?
(R,S)-2-bromopropionitrile + H2O 47% conversion Rhodococcus equi ?
-
 ?
(R,S)-2-chloropropionitrile + H2O 48% conversion Rhodococcus equi ?
-
 ?
(R,S)-2-phenylbutyronitrile + H2O 51% conversion Rhodococcus equi ?
-
 ?
(R,S)-2-phenylpropionitrile + H2O 43% conversion Rhodococcus equi ?
-
 ?
(R,S)-3-oxo-2-phenylbutyronitrile + H2O 43% conversion Rhodococcus equi ?
-
 ?
2-phenylacetonitrile + H2O
-
 Rhodococcus equi 2-phenylpropionamide
-
 ?
3-cyanopyridine + H2O
-
 Rhodococcus equi pyridine-3-carbamide
-
 ?
benzonitrile + H2O
-
 Rhodococcus equi benzoic acid amide
-
 ?
benzonitrile + H2O
-
 Rhodococcus equi TG328-2 benzoic acid amide
-
 ?
isobutyronitrile + H2O
-
 Rhodococcus equi isobutyric acid amide
-
 ?
isobutyronitrile + H2O
-
 Rhodococcus equi TG328-2 isobutyric acid amide
-
 ?
methacrylonitrile + H2O
-
 Rhodococcus equi methacrylamide
-
 ?
additional information besides aromatic and heterocyclic nitriles, aliphatic ones are hydrated preferentially Rhodococcus equi ?
-
 ?
additional information no activity with (R,S)-2-(4-isobutylphenyl)propionitrile and (R,S)-3-(1-cyanoethyl)benzoic acid Rhodococcus equi ?
-
 ?
additional information besides aromatic and heterocyclic nitriles, aliphatic ones are hydrated preferentially Rhodococcus equi TG328-2 ?
-
 ?
additional information no activity with (R,S)-2-(4-isobutylphenyl)propionitrile and (R,S)-3-(1-cyanoethyl)benzoic acid Rhodococcus equi TG328-2 ?
-
 ?
valeronitrile + H2O almost 9000fold higher activity towards valeronitrile compared to (R,S)-2-phenylpropionitrile Rhodococcus equi n-pentanoic acid amide
-
 ?
valeronitrile + H2O almost 9000fold higher activity towards valeronitrile compared to (R,S)-2-phenylpropionitrile Rhodococcus equi TG328-2 n-pentanoic acid amide
-
 ?

Subunits

Subunits Comment Organism
heterodimer 1 * 24000 + 1 * 23000, SDS-PAGE Rhodococcus equi

Synonyms

Synonyms Comment Organism
NHase
-
 Rhodococcus equi

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
-
 Rhodococcus equi

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4 70 highest stability at 4°C in thermal inactivation studies between 4°C and 50°C, the enzyme is inactivated immediately at 70°C Rhodococcus equi

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6 8.5
-
 Rhodococcus equi

Cofactor

Cofactor Comment Organism Structure
pyrroloquinoline quinone
-
 Rhodococcus equi