Activating Compound | Comment | Organism | Structure |
---|---|---|---|
methacrylamide | induces the enzyme | Pseudomonas chlororaphis |
Cloned (Comment) | Organism |
---|---|
genes nhpA and nhpB encoding the alpha and beta subunits of NHase, transcriptional regulation of the nitrile hydratase gene cluster, the NHase gene cluster comprises seven genes: oxdA, amiA, nhpA, nhpB, nhpC, nhpS, and acsA, nhpR codes for a positive transcriptional regulator in the NHase gene cluster, overview | Pseudomonas chlororaphis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Pseudomonas chlororaphis | methacrylamide-induced enzyme activity, no activity in absence of methacrylamide, no or reduced activity in NhpR transcriptional regulator defective mutants | ? | - |
? | |
additional information | Pseudomonas chlororaphis B23 | methacrylamide-induced enzyme activity, no activity in absence of methacrylamide, no or reduced activity in NhpR transcriptional regulator defective mutants | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas chlororaphis | - |
- |
- |
Pseudomonas chlororaphis B23 | - |
- |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
no or reduced activity in NhpR transcriptional regulator defective mutants | Pseudomonas chlororaphis |
23.2 | - |
methacrylamide-induced enzyme activity, no activity in absence of methacrylamide | Pseudomonas chlororaphis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | methacrylamide-induced enzyme activity, no activity in absence of methacrylamide, no or reduced activity in NhpR transcriptional regulator defective mutants | Pseudomonas chlororaphis | ? | - |
? | |
additional information | methacrylamide-induced enzyme activity, no activity in absence of methacrylamide, no or reduced activity in NhpR transcriptional regulator defective mutants | Pseudomonas chlororaphis B23 | ? | - |
? | |
propionitrile + H2O | - |
Pseudomonas chlororaphis | propionamide | - |
? | |
propionitrile + H2O | - |
Pseudomonas chlororaphis B23 | propionamide | - |
? |