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Literature summary for 4.2.1.84 extracted from
- Host plant-dependent metabolism of 4-hydroxybenzylglucosinolate in Pieris rapae: substrate specificity and effects of genetic modification and plant nitrile hydratase (2007), Insect Biochem. Mol. Biol., 37, 1119-1130.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 45 | - |
4-Hydroxyphenylacetonitrile | suggestion from preliminary characterisation of nitrile hydratase activity in crude extracts | Sinapis alba |  |
| 45 | - |
4-Hydroxyphenylacetonitrile | suggestion from preliminary characterisation of nitrile hydratase activity in crude extracts | Sinapis arvensis | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| no activity in Arabidopsis thaliana | - |
strain CYP79A1, genetic modification | - |
| Sinapis alba | - |
- |
- |
| Sinapis arvensis | - |
- |
- |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 11.8 | - |
+/- 6.5, in nmol/min per g fresh weight (foliage) | Sinapis alba |
| 66.6 | - |
+/- 32.0, in nmol/min per g fresh weight (foliage) | Sinapis arvensis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-hydroxyphenylacetonitrile + H2O | - |
Sinapis alba | 4-hydroxyphenylacetamide | - |
? | |
| 4-hydroxyphenylacetonitrile + H2O | - |
Sinapis arvensis | 4-hydroxyphenylacetamide | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| nitrile hydratase | - |
Sinapis alba |
| nitrile hydratase | - |
Sinapis arvensis |
| nitrile hydratase | - |
no activity in Arabidopsis thaliana |
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Release 2023.1 (January 2023)
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