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Literature summary for 4.2.1.84 extracted from

  • Cantarella, M.; Cantarella, L.; Gallifuoco, A.; Frezzini, R.; Spera, A.; Alfani, F.
    A study in UF-membrane reactor on activity and stability of nitrile hydratase from Microbacterium imperiale CBS 498-74 resting cells for propionamide production (2004), J. Mol. Catal. B, 29, 105-113.
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Application

Application Comment Organism
synthesis production of propionamide by use of enzyme in ultrafiltration-membrane reactor with maximum volumetric production of 0.5 g propionamide per litre and h Microbacterium imperiale

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
21.6
-
 Propionitrile pH 7.4, 10°C Microbacterium imperiale

Organism

Organism UniProt Comment Textmining
Microbacterium imperiale
-
-
-
Microbacterium imperiale CBS 498-74
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
11.04
-
 pH 7.4, 10°C Microbacterium imperiale

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
propionitrile + H2O
-
 Microbacterium imperiale propionamide
-
 ?
propionitrile + H2O
-
 Microbacterium imperiale CBS 498-74 propionamide
-
 ?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
5
-
 half-life 38.5 h Microbacterium imperiale
10
-
 half-life 16.5 h Microbacterium imperiale
20
-
 half-life 6.3 h Microbacterium imperiale
25
-
 half-life 3 h Microbacterium imperiale