Application | Comment | Organism |
---|---|---|
synthesis | production of alpha-hydroxy nitriles by use of enzyme | Pseudomonas fluorescens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas fluorescens | - |
- |
- |
Rhodococcus sp. | - |
- |
- |
Rhodococcus sp. | - |
strain ATCC BAA-869 | - |
Rhodococcus sp. | - |
strain ATCC BAA-870 | - |
Rhodococcus sp. | - |
strain DSM 44519 | - |
Rhodococcus sp. Novo SP361 | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-chlorobenzaldehyde + HCN + H2O | - |
Pseudomonas fluorescens | (R)-2-chloromandelonitrile | 90% conversion to alpha-hydroxy nitrile | ? | |
2-fluorobenzaldehyde + HCN + H2O | - |
Pseudomonas fluorescens | ? + H2O | 100% conversion to alpha-hydroxy nitrile | ? | |
2-nitrobenzaldehyde + HCN + H2O | - |
Pseudomonas fluorescens | ? + H2O | 20% conversion to alpha-hydroxy nitrile | ? | |
2-phenylbutyronitrile + H2O | used as well as phenylacetonitrile | Pseudomonas fluorescens | 2-phenylbutyramide | - |
? | |
2-phenylglycinonitrile + H2O | used as well as phenylacetonitrile | Pseudomonas fluorescens | aminoacetamide | - |
? | |
2-phenylpropionitrile + H2O | - |
Rhodococcus sp. | 2-phenylpropionamide | - |
? | |
2-phenylpropionitrile + H2O | used as well as phenylacetonitrile | Pseudomonas fluorescens | 2-phenylpropionamide | - |
? | |
2-phenylpropionitrile + H2O | - |
Rhodococcus sp. Novo SP361 | 2-phenylpropionamide | - |
? | |
3,4-dimethoxybenzonitrile + H2O | second step with amidase (EC 3.5.1.4) | Rhodococcus sp. | 3,4-dimethoxybenzaldehyde + NH3 | - |
? | |
3,4-dimethoxybenzonitrile + H2O | second step with amidase (EC 3.5.1.4) | Rhodococcus sp. Novo SP361 | 3,4-dimethoxybenzaldehyde + NH3 | - |
? | |
3-chlorobenzaldehyde + HCN + H2O | - |
Pseudomonas fluorescens | ? + H2O | 100% conversion to alpha-hydroxy nitrile | ? | |
3-hydroxy-3-phenylpropionitrile + H2O | - |
Rhodococcus sp. | 3-hydroxy-3-phenylpropionamide | - |
? | |
3-hydroxy-3-phenylpropionitrile + H2O | - |
Rhodococcus sp. Novo SP361 | 3-hydroxy-3-phenylpropionamide | - |
? | |
3-phenylpropionitrile + H2O | used as well as phenylacetonitrile | Pseudomonas fluorescens | 3-phenylpropionamide | - |
? | |
4-chlorobenzaldehyde + HCN + H2O | - |
Pseudomonas fluorescens | ? + H2O | 100% conversion to alpha-hydroxy nitrile | ? | |
4-cyanobenzaldehyde + HCN + H2O | - |
Pseudomonas fluorescens | ? + H2O | 100% conversion to alpha-hydroxy nitrile | ? | |
4-hydroxybenzaldehyde + HCN + H2O | - |
Pseudomonas fluorescens | ? + H2O | 55% conversion to alpha-hydroxy nitrile | ? | |
4-methylbenzaldehyde + HCN + H2O | - |
Pseudomonas fluorescens | ? + H2O | 51% conversion to alpha-hydroxy nitrile | ? | |
4-nitrobenzaldehyde + HCN + H2O | - |
Pseudomonas fluorescens | ? + H2O | 100% conversion to alpha-hydroxy nitrile | ? | |
benzaldehyde + HCN | - |
Pseudomonas fluorescens | ? + H2O | 95% conversion to alpha-hydroxy nitrile | ? | |
mandelonitrile + H2O | - |
Rhodococcus sp. | ? | - |
? | |
mandelonitrile + H2O | used as well as phenylacetonitrile | Pseudomonas fluorescens | ? | - |
? | |
N-phenylglycinenitrile + H2O | - |
Rhodococcus sp. | ? | - |
? | |
phenylacetonitrile + H2O | - |
Pseudomonas fluorescens | phenylacetamide | - |
? | |
phenylacetonitrile + H2O | - |
Rhodococcus sp. | phenylacetamide | - |
? | |
phthalonitrile + H2O | - |
Rhodococcus sp. | ? | - |
? |