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Literature summary for 4.2.1.84 extracted from

  • Kobayashi, M.; Shimizu, S.
    Metalloenzyme nitrile hydratase: structure, regulation, and application to biotechnology (1998), Nat. Biotechnol., 16, 733-736.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
light the enzyme activity is increased by exposure to near ultra-violet light Rhodococcus sp.

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Pseudomonas chlororaphis

Metals/Ions

Metals/Ions Comment Organism Structure
Co3+
-
Pseudomonas putida
Fe3+
-
Rhodococcus sp.

Organism

Organism UniProt Comment Textmining
Pseudomonas chlororaphis
-
-
-
Pseudomonas chlororaphis B23
-
-
-
Pseudomonas putida
-
-
-
Rhodococcus rhodochrous
-
-
-
Rhodococcus rhodochrous J1
-
-
-
Rhodococcus sp.
-
-
-
Rhodococcus sp. N-774
-
-
-
Rhodococcus sp. R312
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2(S)-(4-chlorophenyl)-3-methylbutyronitrile + H2O
-
Pseudomonas putida 2(S)-(4-chloro-phenyl)-3-methyl-butyramide
-
?
3-cyanopyridine + H2O
-
Rhodococcus sp. pyridine-3-carbamide
-
?
3-cyanopyridine + H2O
-
Rhodococcus rhodochrous pyridine-3-carbamide i.e. nicotinamide ?
3-cyanopyridine + H2O
-
Rhodococcus sp. N-774 pyridine-3-carbamide
-
?
3-cyanopyridine + H2O
-
Rhodococcus sp. R312 pyridine-3-carbamide
-
?
3-cyanopyridine + H2O
-
Rhodococcus rhodochrous J1 pyridine-3-carbamide i.e. nicotinamide ?
acrylamide + H2O
-
Rhodococcus rhodochrous acrylonitrile
-
?
acrylamide + H2O
-
Rhodococcus sp. acrylonitrile
-
?
acrylamide + H2O
-
Rhodococcus sp. N-774 acrylonitrile
-
?
acrylamide + H2O
-
Rhodococcus sp. R312 acrylonitrile
-
?
acrylamide + H2O
-
Rhodococcus rhodochrous J1 acrylonitrile
-
?

Subunits

Subunits Comment Organism
dimer
-
Pseudomonas putida
dimer
-
Rhodococcus sp.