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Literature summary for 4.2.1.84 extracted from

  • Wu, S.; Fallon, R.D.; Payne, M.S.
    Over-production of stereoselective nitrile hydratase from Pseudomonas putida 5B in Escherichia coli: activity requires a novel downstream protein (1997), Appl. Microbiol. Biotechnol., 48, 704-708.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
P14 protein protein encoded in an open frame of the structural frames that is essential for optimal activity of NHase over-produced in Escherichia coli Pseudomonas putida

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli, strain BL21(DE3) Pseudomonas putida

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
22982
-
1 * 22982, alpha + 1* 24108, beta, amino acid sequence Pseudomonas putida

Organism

Organism UniProt Comment Textmining
Pseudomonas putida
-
-
-
Pseudomonas putida 5B
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2(R)-(4-chlorophenyl)-3-methylbutyronitrile + H2O enantioselective hydration Pseudomonas putida 2(R)-(4-chloro-phenyl)-3-methyl-butyramide
-
?
2(R)-(4-chlorophenyl)-3-methylbutyronitrile + H2O enantioselective hydration Pseudomonas putida 5B 2(R)-(4-chloro-phenyl)-3-methyl-butyramide
-
?
2(S)-(4-chlorophenyl)-3-methylbutyronitrile + H2O
-
Pseudomonas putida 2(S)-(4-chloro-phenyl)-3-methyl-butyramide
-
?
2(S)-(4-chlorophenyl)-3-methylbutyronitrile + H2O
-
Pseudomonas putida 5B 2(S)-(4-chloro-phenyl)-3-methyl-butyramide
-
?
additional information S enantiomer conversion is 14 times greater than the rate of R enantiomer conversion Pseudomonas putida ?
-
?
additional information S enantiomer conversion is 14 times greater than the rate of R enantiomer conversion Pseudomonas putida 5B ?
-
?

Subunits

Subunits Comment Organism
dimer 1 * 22982, alpha + 1* 24108, beta, amino acid sequence Pseudomonas putida