Activating Compound | Comment | Organism | Structure |
---|---|---|---|
P14 protein | protein encoded in an open frame of the structural frames that is essential for optimal activity of NHase over-produced in Escherichia coli | Pseudomonas putida |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli, strain BL21(DE3) | Pseudomonas putida |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
22982 | - |
1 * 22982, alpha + 1* 24108, beta, amino acid sequence | Pseudomonas putida |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas putida | - |
- |
- |
Pseudomonas putida 5B | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2(R)-(4-chlorophenyl)-3-methylbutyronitrile + H2O | enantioselective hydration | Pseudomonas putida | 2(R)-(4-chloro-phenyl)-3-methyl-butyramide | - |
? | |
2(R)-(4-chlorophenyl)-3-methylbutyronitrile + H2O | enantioselective hydration | Pseudomonas putida 5B | 2(R)-(4-chloro-phenyl)-3-methyl-butyramide | - |
? | |
2(S)-(4-chlorophenyl)-3-methylbutyronitrile + H2O | - |
Pseudomonas putida | 2(S)-(4-chloro-phenyl)-3-methyl-butyramide | - |
? | |
2(S)-(4-chlorophenyl)-3-methylbutyronitrile + H2O | - |
Pseudomonas putida 5B | 2(S)-(4-chloro-phenyl)-3-methyl-butyramide | - |
? | |
additional information | S enantiomer conversion is 14 times greater than the rate of R enantiomer conversion | Pseudomonas putida | ? | - |
? | |
additional information | S enantiomer conversion is 14 times greater than the rate of R enantiomer conversion | Pseudomonas putida 5B | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 1 * 22982, alpha + 1* 24108, beta, amino acid sequence | Pseudomonas putida |