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Literature summary for 4.2.1.84 extracted from

  • Kopf, M-A.; Bonnet, D.; Artaud, I.; Pétré, D.; Mansuy, D.
    Key role of alkanoic acids on the spectral properties, activity, and active-site stability of iron-containing nitrile hydratase from Brevibacterium R312 (1996), Eur. J. Biochem., 240, 239-244.
    View publication on PubMed
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Inhibitors

Inhibitors Comment Organism Structure
adipic acid not inhibitory Brevibacterium sp.
hexanoic acid IC50 of 2 mM Brevibacterium sp.
additional information maleic, succinic, glutaric and pimelic acid do not act as inhibitors Brevibacterium sp.
n-butyric acid competitive inhibitor. Complete inhibition beyond 40 mM. IC50 of 4 mM at pH 7.2 in Hepes buffer 100 mM, IC50 of 8 mM at pH 7.2 in sodium phosphate buffer 100 mM, IC50 of 0.8 mM at pH 6.2 in sodium phosphate buffer 100 mM, IC50 of 12 mM at pH 8.2 in sodium phosphate buffer 100 mM Brevibacterium sp.
Valeric acid IC50 of 0.5 mM Brevibacterium sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
17
-
 acrylonitrile pH 7.2, 28ºC, L-NHase Brevibacterium sp.

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ two mol iron/mol of enzyme Brevibacterium sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
95000
-
 4 * 95000 Brevibacterium sp.

Organism

Organism UniProt Comment Textmining
Brevibacterium sp.
-
-
-
Brevibacterium sp. R312
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1325
-
 in the presence of 0.035 mM n-butyric acid Brevibacterium sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acrylonitrile + H2O
-
 Brevibacterium sp. 2-propenoic acid amide
-
 ?
acrylonitrile + H2O
-
 Brevibacterium sp. R312 2-propenoic acid amide
-
 ?

Subunits

Subunits Comment Organism
tetramer 4 * 95000 Brevibacterium sp.

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.9
-
 n-butyric acid pH 7.2, 28ºC Brevibacterium sp.

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.5
-
 IC50 of 0.5 mM Brevibacterium sp. Valeric acid
2
-
 IC50 of 2 mM Brevibacterium sp. hexanoic acid
12
-
 competitive inhibitor. Complete inhibition beyond 40 mM. IC50 of 4 mM at pH 7.2 in Hepes buffer 100 mM, IC50 of 8 mM at pH 7.2 in sodium phosphate buffer 100 mM, IC50 of 0.8 mM at pH 6.2 in sodium phosphate buffer 100 mM, IC50 of 12 mM at pH 8.2 in sodium Brevibacterium sp. n-butyric acid