Activating Compound | Comment | Organism | Structure |
---|---|---|---|
crotonamide | induces high and low molecular mass forms of the enzyme | Rhodococcus rhodochrous | |
cyclohexanecarboxamide | induces the low molecular mass form of the enzyme | Rhodococcus rhodochrous | |
light | the enzyme activity is increased by exposure to near ultra-violet light | Rhodococcus sp. | |
Urea | induces the high molecular mass form of the enzyme | Rhodococcus rhodochrous |
Cloned (Comment) | Organism |
---|---|
- |
Albifimbria verrucaria |
expression in Escherichia coli | Rhodococcus sp. |
high molecular mass-NHase and low molecular mass-NHase genes cloned into Escherichia coli | Rhodococcus rhodochrous |
General Stability | Organism |
---|---|
unstable when diluted, but completely stabilized with low-molecular organic acids such as n-butyric acid, n-valeric acid, propionic acid and acetic acid | Pseudomonas chlororaphis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | high concentrations of acrylonitrile inhibit the enzyme activity | Pseudomonas chlororaphis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.001 | - |
phenylacetonitrile | - |
Agrobacterium tumefaciens | |
0.0079 | - |
indole-3-acetonitrile | - |
Agrobacterium tumefaciens | |
1.89 | - |
acrylonitrile | - |
Rhodococcus rhodochrous |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | - |
Rhodococcus sp. | |
Co2+ | cannot replace ferrous or ferric ions | Pseudomonas chlororaphis | |
Co2+ | induces the enzyme activity | Rhodococcus rhodochrous | |
Co2+ | indispensable for high enzyme activity | Rhodococcus rhodochrous | |
Fe2+ | - |
Rhodococcus rhodochrous | |
Fe2+ | ferrous or ferric ions greatly increase the enzyme activity | Pseudomonas chlororaphis | |
Fe2+ | non-heme iron enzyme with a typical low-spin Fe(III)-active center | Rhodococcus sp. | |
Fe2+ | non-heme iron enzyme with a typical low-spin Fe(III)-active center | Pseudomonas chlororaphis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
27700 | - |
2 * 27700 | Albifimbria verrucaria |
130000 | - |
gel filtration, low molecular mass enzyme, L-NHase | Rhodococcus rhodochrous |
520000 | - |
high molecular mass enzyme, H-NHase | Rhodococcus rhodochrous |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
indole-3-acetonitrile + H2O | Agrobacterium tumefaciens | - |
(indole-3-yl)acetamide | - |
? | |
phenylacetonitrile + 2 H2O | Agrobacterium tumefaciens | - |
phenylacetic acid + NH3 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Agrobacterium tumefaciens | - |
- |
- |
Albifimbria verrucaria | - |
- |
- |
Arthrobacter sp. | - |
- |
- |
Pseudomonas chlororaphis | - |
- |
- |
Pseudomonas chlororaphis B23 | - |
- |
- |
Rhodococcus rhodochrous | - |
- |
- |
Rhodococcus rhodochrous | - |
J1 | - |
Rhodococcus rhodochrous J1 | - |
- |
- |
Rhodococcus rhodochrous J1 | - |
J1 | - |
Rhodococcus sp. | - |
- |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Rhodococcus rhodochrous |
additional information | - |
- |
Rhodococcus sp. |
additional information | - |
141 U/mg of dry cells, Am 324 mutant strain | Pseudomonas chlororaphis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-cyanopyridine + H2O | - |
Rhodococcus sp. | pyridine-3-carbamide | - |
? | |
3-cyanopyridine + H2O | - |
Rhodococcus rhodochrous | pyridine-3-carbamide | i.e. nicotinamide | ? | |
acetonitrile + H2O | - |
Rhodococcus rhodochrous | acetamide | - |
? | |
acetonitrile + H2O | - |
Arthrobacter sp. | acetamide | - |
? | |
acetonitrile + H2O | - |
Rhodococcus sp. | acetamide | - |
? | |
acetonitrile + H2O | - |
Rhodococcus rhodochrous J1 | acetamide | - |
? | |
acrylonitrile + H2O | - |
Rhodococcus rhodochrous | 2-propenoic acid amide | - |
? | |
acrylonitrile + H2O | - |
Arthrobacter sp. | 2-propenoic acid amide | - |
? | |
acrylonitrile + H2O | - |
Rhodococcus sp. | 2-propenoic acid amide | - |
? | |
acrylonitrile + H2O | - |
Pseudomonas chlororaphis | 2-propenoic acid amide | - |
? | |
acrylonitrile + H2O | - |
Pseudomonas chlororaphis B23 | 2-propenoic acid amide | - |
? | |
acrylonitrile + H2O | - |
Rhodococcus rhodochrous J1 | 2-propenoic acid amide | - |
? | |
acrylonitrile + H2O | - |
Rhodococcus rhodochrous | acrylamide | - |
? | |
cyanamide + H2O | - |
Albifimbria verrucaria | urea | - |
? | |
hydroxyacetonitrile + H2O | - |
Arthrobacter sp. | hydroxyacetamide | - |
? | |
indole-3-acetonitrile + H2O | - |
Agrobacterium tumefaciens | (indole-3-yl)acetamide | - |
? | |
methacrylamide + H2O | causes the greatest induction of activity | Pseudomonas chlororaphis | ? | - |
? | |
methacrylamide + H2O | causes the greatest induction of activity | Pseudomonas chlororaphis B23 | ? | - |
? | |
additional information | - |
Pseudomonas chlororaphis | ? | - |
? | |
additional information | the enzyme acts on low-molecular aliphatic nitriles with 2-5 carbons but not on aliphatic nitriles with more than 6 carbons | Arthrobacter sp. | ? | - |
? | |
additional information | H-NHase acts preferentially on aliphatic nitriles, while L-NHase has a higher affinity for aromatic nitriles | Rhodococcus rhodochrous | ? | - |
? | |
additional information | - |
Pseudomonas chlororaphis B23 | ? | - |
? | |
additional information | H-NHase acts preferentially on aliphatic nitriles, while L-NHase has a higher affinity for aromatic nitriles | Rhodococcus rhodochrous J1 | ? | - |
? | |
n-butyronitrile + H2O | - |
Rhodococcus rhodochrous | n-butyric acid amide | - |
? | |
n-butyronitrile + H2O | - |
Arthrobacter sp. | n-butyric acid amide | - |
? | |
n-butyronitrile + H2O | - |
Rhodococcus sp. | n-butyric acid amide | - |
? | |
n-butyronitrile + H2O | - |
Pseudomonas chlororaphis | n-butyric acid amide | - |
? | |
n-butyronitrile + H2O | - |
Pseudomonas chlororaphis B23 | n-butyric acid amide | - |
? | |
n-butyronitrile + H2O | - |
Rhodococcus rhodochrous J1 | n-butyric acid amide | - |
? | |
phenylacetonitrile + 2 H2O | - |
Agrobacterium tumefaciens | phenylacetic acid + NH3 | - |
? | |
propionitrile + H2O | - |
Rhodococcus rhodochrous | propionic acid amide | - |
? | |
propionitrile + H2O | - |
Arthrobacter sp. | propionic acid amide | - |
? | |
propionitrile + H2O | - |
Rhodococcus sp. | propionic acid amide | - |
? | |
propionitrile + H2O | - |
Pseudomonas chlororaphis | propionic acid amide | - |
? | |
propionitrile + H2O | - |
Pseudomonas chlororaphis B23 | propionic acid amide | - |
? | |
propionitrile + H2O | - |
Rhodococcus rhodochrous J1 | propionic acid amide | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | - |
Rhodococcus sp. |
dimer | 2 * 27700 | Albifimbria verrucaria |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
5 | - |
- |
Rhodococcus rhodochrous |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
the enzyme is heat-stable | Rhodococcus rhodochrous |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Rhodococcus rhodochrous |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyrroloquinoline quinone | - |
Pseudomonas chlororaphis |