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Literature summary for 4.2.1.84 extracted from

  • Stevens, J.M.; Rao Saroja, N.; Jaouen, M.; Belghazi, M.; Schmitter, J-M.; Mansuy, D.; Artaud, I.; Sari, M-A.
    Chaperone-assisted expression, purification, and characterization of recombinant nitrile hydratase NI1 from Comamonas testosteroni (2003), Protein Expr. Purif., 29, 70-76.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Comamonas testosteroni

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.25
-
2-nitro-5-thiocyanato-benzoic acid pH 7.2, 28ºC, recombinant enzyme Comamonas testosteroni
0.6
-
2-nitro-5-thiocyanato-benzoic acid pH 7.2, 28ºC, native enzyme Comamonas testosteroni
2.5
-
Cyanovaleric acid pH 7.2, 28ºC, native enzyme Comamonas testosteroni
3.2
-
Cyanovaleric acid pH 7.2, 28ºC, recombinant enzyme Comamonas testosteroni

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ ferric citrate has no effect on the enzyme activity Comamonas testosteroni

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
22700
-
2 * 22800, alpha + 2 * 22700, beta, SDS-PAGE Comamonas testosteroni
22800
-
2 * 22800, alpha + 2 * 22700, beta, SDS-PAGE Comamonas testosteroni
84000
-
gel filtration Comamonas testosteroni

Organism

Organism UniProt Comment Textmining
Comamonas testosteroni
-
-
-

Purification (Commentary)

Purification (Comment) Organism
chromatography on Q-Sepharose, phenyl-Sepharose and Uno Q Comamonas testosteroni

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.8
-
recombinant enzyme, with 2-nitro-5-thiocyanato-benzoic acid Comamonas testosteroni
7.7
-
native enzyme, with 2-nitro-5-thiocyanato-benzoic acid Comamonas testosteroni
95
-
native enzyme, with cyanovaleric acid Comamonas testosteroni
100
-
recombinant enzyme, with cyanovaleric acid Comamonas testosteroni

Storage Stability

Storage Stability Organism
-40ºC, pH 7.2, 40 mM butyric acid, stable Comamonas testosteroni

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-nitro-5-thiocyanato-benzoic acid + H2O
-
Comamonas testosteroni ?
-
?
cyanovaleric acid + H2O
-
Comamonas testosteroni ?
-
?

Subunits

Subunits Comment Organism
?
-
Comamonas testosteroni
tetramer 2 * 22800, alpha + 2 * 22700, beta, SDS-PAGE Comamonas testosteroni