Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Comamonas testosteroni |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.25 | - |
2-nitro-5-thiocyanato-benzoic acid | pH 7.2, 28ºC, recombinant enzyme | Comamonas testosteroni | |
0.6 | - |
2-nitro-5-thiocyanato-benzoic acid | pH 7.2, 28ºC, native enzyme | Comamonas testosteroni | |
2.5 | - |
Cyanovaleric acid | pH 7.2, 28ºC, native enzyme | Comamonas testosteroni | |
3.2 | - |
Cyanovaleric acid | pH 7.2, 28ºC, recombinant enzyme | Comamonas testosteroni |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | ferric citrate has no effect on the enzyme activity | Comamonas testosteroni |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
22700 | - |
2 * 22800, alpha + 2 * 22700, beta, SDS-PAGE | Comamonas testosteroni |
22800 | - |
2 * 22800, alpha + 2 * 22700, beta, SDS-PAGE | Comamonas testosteroni |
84000 | - |
gel filtration | Comamonas testosteroni |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Comamonas testosteroni | - |
- |
- |
Purification (Comment) | Organism |
---|---|
chromatography on Q-Sepharose, phenyl-Sepharose and Uno Q | Comamonas testosteroni |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
6.8 | - |
recombinant enzyme, with 2-nitro-5-thiocyanato-benzoic acid | Comamonas testosteroni |
7.7 | - |
native enzyme, with 2-nitro-5-thiocyanato-benzoic acid | Comamonas testosteroni |
95 | - |
native enzyme, with cyanovaleric acid | Comamonas testosteroni |
100 | - |
recombinant enzyme, with cyanovaleric acid | Comamonas testosteroni |
Storage Stability | Organism |
---|---|
-40ºC, pH 7.2, 40 mM butyric acid, stable | Comamonas testosteroni |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-nitro-5-thiocyanato-benzoic acid + H2O | - |
Comamonas testosteroni | ? | - |
? | |
cyanovaleric acid + H2O | - |
Comamonas testosteroni | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | - |
Comamonas testosteroni |
tetramer | 2 * 22800, alpha + 2 * 22700, beta, SDS-PAGE | Comamonas testosteroni |