Application | Comment | Organism |
---|---|---|
synthesis | - |
Pseudomonas chlororaphis |
Crystallization (Comment) | Organism |
---|---|
- |
Pseudomonas chlororaphis |
General Stability | Organism |
---|---|
completely stabilized by 22 mM n-butyric acid | Pseudomonas chlororaphis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,10-phenanthroline | 1-5 mM | Pseudomonas chlororaphis | |
2,2'-dipyridyl | 1-5 mM | Pseudomonas chlororaphis | |
8-hydroxyquinoline | - |
Pseudomonas chlororaphis | |
Ag+ | 0.01-1 mM AgNO3 | Pseudomonas chlororaphis | |
Cu2+ | 1-5 mM CuSO4 | Pseudomonas chlororaphis | |
cysteamine | 1 mM | Pseudomonas chlororaphis | |
Disodium 4,5-dihydroxy-m-benzene disulfonate | 0.1-5 mM | Pseudomonas chlororaphis | |
FeCl3 | 1-5 mM | Pseudomonas chlororaphis | |
FeSO4 | 1-5 mM, weak inhibition | Pseudomonas chlororaphis | |
H2O2 | 0.1 mM | Pseudomonas chlororaphis | |
Hg2+ | HgCl2, 0.01-1 mM | Pseudomonas chlororaphis | |
hydroxylamine | 1 mM | Pseudomonas chlororaphis | |
NaN3 | 1 mM | Pseudomonas chlororaphis | |
p-chloromercuribenzoate | 0.1 mM | Pseudomonas chlororaphis | |
PbCl2 | 1 mM | Pseudomonas chlororaphis | |
Penicillamine | 1 mM; L- and D-isomer | Pseudomonas chlororaphis | |
phenylhydrazine | 1 mM | Pseudomonas chlororaphis | |
Semicarbazide | 1 mM | Pseudomonas chlororaphis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.03 | - |
n-butyronitrile | pH 7.0, 20ºC | Pseudomonas chlororaphis | |
2.33 | - |
n-valeronitrile | pH 7.0, 20ºC | Pseudomonas chlororaphis | |
3.8 | - |
Methacrylonitrile | - |
Pseudomonas chlororaphis | |
22.2 | - |
Chloroacetonitrile | pH 7.0, 20ºC | Pseudomonas chlororaphis | |
29.4 | - |
Propionitrile | pH 7.0, 20ºC | Pseudomonas chlororaphis | |
34.6 | - |
acrylonitrile | pH 7.0, 20ºC | Pseudomonas chlororaphis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | contains approximately 4 mol iron/mol enzyme | Pseudomonas chlororaphis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
25000 | - |
4 * 25000, SDS-PAGE | Pseudomonas chlororaphis |
100000 | - |
gel filtration | Pseudomonas chlororaphis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas chlororaphis | - |
- |
- |
Pseudomonas chlororaphis B23 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate fractionation, chromatography on DEAE-Sephacel, octyl and phenyl-Sepharose CL-4B | Pseudomonas chlororaphis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Pseudomonas chlororaphis |
1840 | - |
crystallized enzyme | Pseudomonas chlororaphis |
Storage Stability | Organism |
---|---|
-20°C, 50% glycerol, 0.01 M HEPES/KOH buffer, pH 7.2, 22 mM n-butyric acid, stable for more than 2 months | Pseudomonas chlororaphis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acrylonitrile + H2O | - |
Pseudomonas chlororaphis | 2-propenoic acid amide | i.e. acrylic acid amide | ? | |
chloroacetonitrile + H2O | - |
Pseudomonas chlororaphis | chloroacetamide | - |
? | |
chloroacetonitrile + H2O | - |
Pseudomonas chlororaphis B23 | chloroacetamide | - |
? | |
isobutyronitrile + H2O | - |
Pseudomonas chlororaphis | isobutyric acid amide | - |
? | |
methacrylonitrile + H2O | - |
Pseudomonas chlororaphis | methylacrylic acid amide | - |
? | |
additional information | specificity overview | Pseudomonas chlororaphis | ? | - |
? | |
additional information | aromatic nitriles are barely hydrated by the enzyme | Pseudomonas chlororaphis | ? | - |
? | |
additional information | formamide, acetamide, acrylamide, propionamide, n-butyramide, isobutyramide, n-valeramide, succinamide, benzamide, phenylacetamide and lactamide are no substrates | Pseudomonas chlororaphis | ? | - |
? | |
additional information | specificity overview | Pseudomonas chlororaphis B23 | ? | - |
? | |
additional information | aromatic nitriles are barely hydrated by the enzyme | Pseudomonas chlororaphis B23 | ? | - |
? | |
additional information | formamide, acetamide, acrylamide, propionamide, n-butyramide, isobutyramide, n-valeramide, succinamide, benzamide, phenylacetamide and lactamide are no substrates | Pseudomonas chlororaphis B23 | ? | - |
? | |
n-butyronitrile + H2O | - |
Pseudomonas chlororaphis | n-butyric acid amide | - |
? | |
n-capronitrile + H2O | - |
Pseudomonas chlororaphis | n-hexanoic acid amide | - |
? | |
propionitrile + H2O | - |
Pseudomonas chlororaphis | propionic acid amide | - |
? | |
propionitrile + H2O | - |
Pseudomonas chlororaphis B23 | propionic acid amide | - |
? | |
valeronitrile + H2O | - |
Pseudomonas chlororaphis | n-pentanoic acid amide | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 25000, SDS-PAGE | Pseudomonas chlororaphis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | - |
with propionitrile and acrylonitrile as substrates | Pseudomonas chlororaphis |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
10 | 30 | 79% of activity maximum at 10°C, 13% of activity maximum at 30°C | Pseudomonas chlororaphis |
25 | 50 | about 50% of activity maximum at 25°C, about 70% of activity maximum at 50°C | Pseudomonas chlororaphis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
10 | - |
10 min, 22 mM n-butyric acid, stable | Pseudomonas chlororaphis |
20 | - |
10 min, 22 mM n-butyric acid, stable | Pseudomonas chlororaphis |
25 | - |
10 min, 22 mM n-butyric acid, 12.5% loss of activity | Pseudomonas chlororaphis |
30 | - |
10 min, 22 mM n-butyric acid, 25% loss of activity | Pseudomonas chlororaphis |
35 | - |
1 hour, 10 mM methacrylonitrile stable up to this temperature | Pseudomonas chlororaphis |
35 | - |
10 min, 22 mM n-butyric acid, 46.6% loss of activity | Pseudomonas chlororaphis |
40 | - |
10 min, 22 mM n-butyric acid, 94.3% loss of activity | Pseudomonas chlororaphis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
30.7 | - |
Propionitrile | - |
Pseudomonas chlororaphis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Pseudomonas chlororaphis |