General Stability | Organism |
---|---|
organic acids stabilize, stable for more than 1 month in 0.1 M HEPES/KOH, pH 7.2, with 44 mM n-butyric acid, n-valeric acid, isovaleric acid, isobutyric acid or n-caproic acid | Rhodococcus rhodochrous |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ag+ | 0.01-1 mM AgNO3 | Rhodococcus rhodochrous | |
diethyldithiocarbamate | 0.1 mM | Rhodococcus rhodochrous | |
Hg2+ | HgCl2, 0.01-1 mM | Rhodococcus rhodochrous | |
hydroxylamine | 1 mM | Rhodococcus rhodochrous | |
KCN | 0.01 mM | Rhodococcus rhodochrous | |
Na2S2O4 | 1 mM | Rhodococcus rhodochrous | |
phenylhydrazine | 1 mM | Rhodococcus rhodochrous | |
Semicarbazide | 1 mM, weak inhibition | Rhodococcus rhodochrous |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.85 | - |
Isobutyronitrile | pH 7.0, 20ºC, H-NHase | Rhodococcus rhodochrous | |
1.89 | - |
acrylonitrile | pH 7.0, 20ºC, H-NHase | Rhodococcus rhodochrous | |
1.92 | - |
Propionitrile | pH 7.0, 20ºC, H-NHase | Rhodococcus rhodochrous | |
4.88 | - |
Crotononitrile | pH 7.0, 20ºC, H-NHase | Rhodococcus rhodochrous | |
6.76 | - |
Methacrylonitrile | pH 7.0, 20ºC | Rhodococcus rhodochrous | |
10 | - |
Cyanopyrazine | pH 7.0, 20ºC | Rhodococcus rhodochrous | |
12.2 | - |
Chloroacetonitrile | pH 7.0, 20ºC, H-NHase | Rhodococcus rhodochrous | |
18.5 | - |
4-cyanopyridine | pH 7.0, 20ºC, H-NHase | Rhodococcus rhodochrous | |
21.7 | - |
2-Cyanopyridine | pH 7.0, 20ºC, H-NHase | Rhodococcus rhodochrous | |
21.7 | - |
n-butyronitrile | pH 7.0, 20ºC, H-NHase | Rhodococcus rhodochrous | |
28.6 | - |
Benzonitrile | pH 7.0, 20ºC, H-NHase | Rhodococcus rhodochrous | |
200 | - |
3-Cyanopyridine | pH 7.0, 20ºC, H-NHase | Rhodococcus rhodochrous |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | contains approximately 11-12 mol cobalt/mol enzyme | Rhodococcus rhodochrous |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
26000 | - |
x * 26000, alpha, + x * 29000, beta, SDS-PAGE | Rhodococcus rhodochrous |
29000 | - |
x * 26000, alpha, + x * 29000, beta, SDS-PAGE | Rhodococcus rhodochrous |
505000 | 530000 | sedimentation equilibrium method, gel permeation HPLC | Rhodococcus rhodochrous |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rhodococcus rhodochrous | - |
2 kinds of enzyme, a high molecular mass: H-NHase, and a low molecular mass enzyme: L-NHase | - |
Rhodococcus rhodochrous J1 | - |
2 kinds of enzyme, a high molecular mass: H-NHase, and a low molecular mass enzyme: L-NHase | - |
Purification (Comment) | Organism |
---|---|
- |
Rhodococcus rhodochrous |
ammonium sulfate fractionation, chromatography on DEAE-Sephacel, phenyl-Sepharose and Sephacryl S-300, 3.31fold purification | Rhodococcus rhodochrous |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Rhodococcus rhodochrous |
371 | - |
purified enzyme | Rhodococcus rhodochrous |
Storage Stability | Organism |
---|---|
organic acids stabilize, stable for more than 1 month in 0.1 M HEPES/KOH, pH 7.2, with 44 mM n-butyric acid, n-valeric acid, isovaleric acid, isobutyric acid or n-caproic acid | Rhodococcus rhodochrous |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-cyanopyridine + H2O | - |
Rhodococcus rhodochrous | pyridine-2-carbamide | - |
? | |
3-cyanopyridine + H2O | - |
Rhodococcus rhodochrous | pyridine-3-carbamide | i.e. nicotinamide | ? | |
4-cyanopyridine + H2O | - |
Rhodococcus rhodochrous | isonicotinamide | - |
? | |
acetonitrile + H2O | best substrate | Rhodococcus rhodochrous | acetamide | - |
? | |
acetonitrile + H2O | best substrate | Rhodococcus rhodochrous J1 | acetamide | - |
? | |
acrylonitrile + H2O | - |
Rhodococcus rhodochrous | 2-propenoic acid amide | i.e. acrylic acid amide | ? | |
benzonitrile + H2O | - |
Rhodococcus rhodochrous | benzoic acid amide | - |
? | |
chloroacetonitrile + H2O | - |
Rhodococcus rhodochrous | chloroacetamide | - |
? | |
chloroacetonitrile + H2O | - |
Rhodococcus rhodochrous J1 | chloroacetamide | - |
? | |
crotononitrile + H2O | 19% of the activity with propionitrile | Rhodococcus rhodochrous | (E)-2-butenoic acid amide | - |
? | |
cyanopyrazine + H2O | - |
Rhodococcus rhodochrous | pyrazincarbamide | - |
? | |
cyclopropylcyanide + H2O | - |
Rhodococcus rhodochrous | ? | - |
? | |
ethylene cyanhydrine + H2O | - |
Rhodococcus rhodochrous | ? | - |
? | |
isobutyronitrile + H2O | - |
Rhodococcus rhodochrous | isobutyramide | - |
? | |
methacrylonitrile + H2O | - |
Rhodococcus rhodochrous | methylacrylic acid amide | - |
? | |
additional information | specificity overview | Rhodococcus rhodochrous | ? | - |
? | |
additional information | specificity overview | Rhodococcus rhodochrous J1 | ? | - |
? | |
n-butyronitrile + H2O | - |
Rhodococcus rhodochrous | n-butyric acid amide | - |
? | |
n-butyronitrile + H2O | - |
Rhodococcus rhodochrous J1 | n-butyric acid amide | - |
? | |
propionitrile + H2O | - |
Rhodococcus rhodochrous | propionic acid amide | - |
? | |
propionitrile + H2O | - |
Rhodococcus rhodochrous J1 | propionic acid amide | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 26000, alpha, + x * 29000, beta, SDS-PAGE | Rhodococcus rhodochrous |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
- |
Rhodococcus rhodochrous |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
stable up to, 30 min, pH 7.0, 44 mM n-butyric acid | Rhodococcus rhodochrous |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | 6.8 | - |
Rhodococcus rhodochrous |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 8 | about 50% of activity maximum at pH 5, about 60% of activity maximum at pH 8 | Rhodococcus rhodochrous |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6 | 9 | 20°C, 60 min, stable in this range, rapid loss of activity below pH 6.0 and above pH 9.0 | Rhodococcus rhodochrous |
6 | 9 | 25°C, 10 min, stable in this range, rapid loss of activity below pH 6.0 and above pH 9.0 | Rhodococcus rhodochrous |