Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 4.2.1.84 extracted from

  • Moreau, J.L.; Azza, S.; Arnaud, A.; Galzy, P.
    Purification and characterization of nitrile hydratase of a mutant strain of Brevibacterium sp. (1993), J. Basic Microbiol., 33, 323-329.
No PubMed abstract available
Search for more literature for 4.2.1.84

Inhibitors

Inhibitors Comment Organism Structure
Adipamic acid
-
 Brevibacterium sp.
adipamide
-
 Brevibacterium sp.
adipic acid
-
 Brevibacterium sp.
Ca2+ weak Brevibacterium sp.
Cu2+ 85% inhibition at 10 mM Brevibacterium sp.
Cyanovaleramide
-
 Brevibacterium sp.
Cyanovaleric acid
-
 Brevibacterium sp.
diisopropylfluorophosphate 0.05 mM, 100% inhibition Brevibacterium sp.
Glutaraldehyde 0.05 mM, 58% inhibition Brevibacterium sp.
Hg2+ 10 mM, 90% inhibition Brevibacterium sp.
iodoacetic acid at 0.05 mM, 27% inhibition Brevibacterium sp.
Mn2+
-
 Brevibacterium sp.
N,N-Dimethylacetamide
-
 Brevibacterium sp.
N-bromosuccinimide 100% inhibition at 0.005 mM Brevibacterium sp.
NEM 68% inhibition at 0.05 mM Brevibacterium sp.
Thioacetamide
-
 Brevibacterium sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information
-
 Brevibacterium sp.
8
-
 Propionitrile R312 strain Brevibacterium sp.
21
-
 Cyanovaleric acid ACV2 mutant Brevibacterium sp.
28
-
 Cyanovaleric acid R312 strain Brevibacterium sp.
30
-
 adiponitrile R312 strain Brevibacterium sp.
38
-
 adiponitrile ACV2 mutant Brevibacterium sp.
50
-
 Propionitrile ACV2 mutant Brevibacterium sp.
156
-
 Cyanovaleramide R312 strain Brevibacterium sp.
370
-
 Cyanovaleramide ACV2 mutant Brevibacterium sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
26000
-
 x * 26000 + x * 27500, SDS-PAGE Brevibacterium sp.
27500
-
 x * 26000 + x * 27500, SDS-PAGE Brevibacterium sp.
80000
-
 gel filtration Brevibacterium sp.

Organism

Organism UniProt Comment Textmining
Brevibacterium sp.
-
-
-
Brevibacterium sp.
-
 mutant strain ACV2
-
Brevibacterium sp. R312
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation, ion exchange chromatography and chromatography on phenyl-Sepharose CL-4B, 11fold purification, mutant strain ACV2 Brevibacterium sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1600
-
 purified enzyme Brevibacterium sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
adiponitrile + 2 H2O
-
 Brevibacterium sp. adipic acid amide
-
 ?
adiponitrile + 2 H2O
-
 Brevibacterium sp. R312 adipic acid amide
-
 ?
cyanovaleramide
-
 Brevibacterium sp. valerodinitrile + H2O
-
 ?
cyanovaleramide
-
 Brevibacterium sp. R312 valerodinitrile + H2O
-
 ?
cyanovaleric acid + H2O
-
 Brevibacterium sp. ?
-
 ?
cyanovaleric acid + H2O
-
 Brevibacterium sp. R312 ?
-
 ?
additional information wide substrate spectrum Brevibacterium sp. ?
-
 ?
additional information wide substrate spectrum Brevibacterium sp. R312 ?
-
 ?
propionitrile + H2O
-
 Brevibacterium sp. propionic acid amide
-
 ?
propionitrile + H2O
-
 Brevibacterium sp. R312 propionic acid amide
-
 ?

Subunits

Subunits Comment Organism
? x * 26000 + x * 27500, SDS-PAGE Brevibacterium sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
35
-
-
 Brevibacterium sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
45
-
 5 min, complete loss of activity Brevibacterium sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
 Brevibacterium sp.

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.35
-
 Cyanovaleric acid R312 strain Brevibacterium sp.
2
-
 N,N-Dimethylacetamide R312 strain Brevibacterium sp.
2
-
 N,N-Dimethylacetamide ACV2 mutant Brevibacterium sp.
3.8
-
 Adipamic acid ACV2 mutant Brevibacterium sp.
5
-
 Cyanovaleric acid ACV2 mutant Brevibacterium sp.
12
-
 Cyanovaleramide R312 strain Brevibacterium sp.
14.5
-
 adipamide ACV2 mutant Brevibacterium sp.
31
-
 adipamide R312 strain Brevibacterium sp.
35
-
 adipic acid R312 strain Brevibacterium sp.
43
-
 Adipamic acid R312 strain Brevibacterium sp.
73
-
 Cyanovaleramide ACV2 mutant Brevibacterium sp.
100
-
 Thioacetamide R312 strain Brevibacterium sp.
110
-
 Thioacetamide ACV2 mutant Brevibacterium sp.
220
-
 adipic acid ACV2 mutant Brevibacterium sp.