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Literature summary for 4.2.1.84 extracted from
- Formation and purification of nitrile hydratase from Corynebacterium pseudodiphteritium ZBB-41 (1992), Appl. Biochem. Biotechnol., 36, 171-181.
No PubMed abstract available
Application
| Application | Comment | Organism |
|---|---|---|
| synthesis | useful for acrylamide production | Corynebacterium pseudodiphtheriticum |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ag+ | strong inhibition | Corynebacterium pseudodiphtheriticum |  |
| Cu2+ | strong inhibition | Corynebacterium pseudodiphtheriticum | |
| EDTA | strong inhibition | Corynebacterium pseudodiphtheriticum | |
| Hg2+ | strong inhibition | Corynebacterium pseudodiphtheriticum | |
| iodoacetic acid | - |
Corynebacterium pseudodiphtheriticum | |
| NaCN | non-competitive inhibition | Corynebacterium pseudodiphtheriticum | |
| NaN3 | - |
Corynebacterium pseudodiphtheriticum | |
| phenylmercuric acetate | strong inhibition | Corynebacterium pseudodiphtheriticum | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.21 | - |
acrylonitrile | - |
Corynebacterium pseudodiphtheriticum | |
| 210 | - |
acrylonitrile | pH 7.5, 25°C | Corynebacterium pseudodiphtheriticum | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 25000 | - |
x * 25000 + x * 28000, SDS-PAGE | Corynebacterium pseudodiphtheriticum |
| 28000 | - |
x * 25000 + x * 28000, SDS-PAGE | Corynebacterium pseudodiphtheriticum |
| 80000 | - |
gradient gel electrophoresis | Corynebacterium pseudodiphtheriticum |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Corynebacterium pseudodiphtheriticum | - |
- |
- |
| Corynebacterium pseudodiphtheriticum ZBB-41 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| to homogeneity, using several chromatographic steps, including DEAE, hydroxyapatite, and Sephadex G-200 column chromatography | Corynebacterium pseudodiphtheriticum |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
effect of different organic and inorganic compounds on activity | Corynebacterium pseudodiphtheriticum |
| 315.2 | - |
- |
Corynebacterium pseudodiphtheriticum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acrylonitrile + H2O | - |
Corynebacterium pseudodiphtheriticum | 2-propenoic acid amide | i.e. acrylic acid amide | ? | |
| acrylonitrile + H2O | - |
Corynebacterium pseudodiphtheriticum ZBB-41 | 2-propenoic acid amide | i.e. acrylic acid amide | ? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 25000 + x * 28000, SDS-PAGE | Corynebacterium pseudodiphtheriticum |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
- |
Corynebacterium pseudodiphtheriticum |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | - |
90 min, 60% loss of activity | Corynebacterium pseudodiphtheriticum |
| 50 | - |
90 min, complete loss of activity | Corynebacterium pseudodiphtheriticum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
- |
Corynebacterium pseudodiphtheriticum |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | 8 | 25°C, 90 min, stable | Corynebacterium pseudodiphtheriticum |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.13 | - |
NaCN | pH 7.5, 25°C, non-competitive with acrylonitrile | Corynebacterium pseudodiphtheriticum | |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Corynebacterium pseudodiphtheriticum | polyacrylamide isoelectrofocusing | - |
4.2 |
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