Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 4.2.1.83 extracted from

  • Hara, H.; Masai, E.; Katayama, Y.; Fukuda, M.
    The 4-oxalomesaconate hydratase gene, involved in the protocatechuate 4,5-cleavage pathway, is essential to vanillate and syringate degradation in Sphingomonas paucimobilis SYK-6 (2000), J. Bacteriol., 182, 6950-6957.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.2.1.83

Activating Compound

Activating Compound Comment Organism Structure
cysteine
-
 Sphingomonas paucimobilis
DTT
-
 Sphingomonas paucimobilis
reduced glutathione
-
 Sphingomonas paucimobilis

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Sphingomonas paucimobilis

Inhibitors

Inhibitors Comment Organism Structure
HgCl2
-
 Sphingomonas paucimobilis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.138
-
 (E)-3-Carboxy-5-oxo-2-hex-2-enedioic acid pH 8.0, 30°C Sphingomonas paucimobilis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
38000
-
 2 * 38000 Sphingomonas paucimobilis
69500
-
 gel filtration Sphingomonas paucimobilis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Sphingomonas paucimobilis enzyme is essential for catabolism of both vanillate and syringate ?
-
 ?

Organism

Organism UniProt Comment Textmining
Sphingomonas paucimobilis Q9KWL6
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme Sphingomonas paucimobilis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
557
-
-
 Sphingomonas paucimobilis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(E)-3-carboxy-5-oxo-2-hex-2-enedioic acid + H2O
-
 Sphingomonas paucimobilis 2-hydroxy-4-oxobutane-1,2,4-tricarboxylate
-
 ?
additional information enzyme is essential for catabolism of both vanillate and syringate Sphingomonas paucimobilis ?
-
 ?

Subunits

Subunits Comment Organism
homodimer 2 * 38000 Sphingomonas paucimobilis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
-
 Sphingomonas paucimobilis

pI Value

Organism Comment pI Value Maximum pI Value
Sphingomonas paucimobilis isoelectric focusing
-
 4.9