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Literature summary for 4.2.1.82 extracted from
- Characterization of D-xylonate dehydratase YjhG from Escherichia coli (2015), Bioengineered, 6, 227-232 .
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2-mercaptoethanol | 1 mM, 44% loss of activity | Escherichia coli |  |
| dithiothreitol | 1 mM, 70% loss of activity | Escherichia coli | |
| Ni2+ | 5 mM | Escherichia coli | |
| Zn2+ | 5 mM | Escherichia coli | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 4.88 | - |
D-xylonate | pH 8.0, 30°C | Escherichia coli | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | 5 mM, required for activity, activates | Escherichia coli | |
| Mn2+ | 5 mM, required for activity, activates | Escherichia coli | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 70000 | - |
SDS-PAGE | Escherichia coli |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-xylonate | Escherichia coli | - |
2-dehydro-3-deoxy-D-arabinonate + H2O | - |
? | |
| D-xylonate | Escherichia coli K12 | - |
2-dehydro-3-deoxy-D-arabinonate + H2O | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | P39358 | - |
- |
| Escherichia coli K12 | P39358 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-xylonate | - |
Escherichia coli | 2-dehydro-3-deoxy-D-arabinonate + H2O | - |
? | |
| D-xylonate | - |
Escherichia coli K12 | 2-dehydro-3-deoxy-D-arabinonate + H2O | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| YjhG | - |
Escherichia coli |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
- |
Escherichia coli |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | 42 | 20°C: about 60% of maximal activity, 42°C: about 50% of maximal activity, activity is almost undetectable above 60x01C° | Escherichia coli |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | - |
the enzyme is denatured to precipitation above 70°C | Escherichia coli |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
- |
Escherichia coli |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6.7 | 8.5 | pH 6.7: about 65% of maximal activity, pH 8.5: about 65% of maximal activity | Escherichia coli |
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