Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 4.2.1.81 extracted from

  • Yew, W.S.; Fedorov, A.A.; Fedorov, E.V.; Wood, B.M.; Almo, S.C.; Gerlt, J.A.
    Evolution of enzymatic activities in the enolase superfamily: D-tartrate dehydratase from Bradyrhizobium japonicum (2006), Biochemistry, 45, 14598-14608.
    View publication on PubMed
Search for more literature for 4.2.1.81

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli strain BL21 Bradyrhizobium japonicum

Crystallization (Commentary)

Crystallization (Comment) Organism
all crystals, wild type TarD liganded with Mg2+, selenomethionine-substituted TarD liganded with Mg2+, wild-type TarD liganded with Mg2+ and meso-tartrate, and the K184A mutant liganded with Mg2+ and D-tartrate, are grown by the hanging drop method at room temperature Bradyrhizobium japonicum

Protein Variants

Protein Variants Comment Organism
H322A kcatKm is 193000fold lower than wild-type value Bradyrhizobium japonicum
H322N kcatKm is 607fold lower than wild-type value Bradyrhizobium japonicum
H322Q kcatKm is 340000fold lower than wild-type value Bradyrhizobium japonicum
K102A inactive mutant protein Bradyrhizobium japonicum
K102M inactive mutant protein Bradyrhizobium japonicum
K184A inactive mutant enzyme Bradyrhizobium japonicum
K184R reduced activity. kcat/Km is 500fold lower than wild-type value Bradyrhizobium japonicum

Inhibitors

Inhibitors Comment Organism Structure
meso-tartrate competitive inhibitor Bradyrhizobium japonicum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.035
-
 (S,S)-Tartrate 25°C, pH 7.5, mutant enzyme K184R Bradyrhizobium japonicum
0.048
-
 (S,S)-Tartrate 25°C, pH 7.5, mutant enzyme H322N Bradyrhizobium japonicum
0.086
-
 (S,S)-Tartrate 25°C, pH 7.5, wild-type enzyme Bradyrhizobium japonicum
6.2
-
 (S,S)-Tartrate 25°C, pH 7.5, mutant enzyme H322A Bradyrhizobium japonicum
7.7
-
 (S,S)-Tartrate 25°C, pH 7.5, mutant enzyme H322Q Bradyrhizobium japonicum

Organism

Organism UniProt Comment Textmining
Bradyrhizobium japonicum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Bradyrhizobium japonicum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S,S)-tartrate mechanism in which Lys 184 initiates the reaction by abstraction of the R-proton to generate a Mg2+-stabilized enediolate intermediate, and the vinylogous beta-elimination of the 3-OH group is general acid-catalyzed by the His 322, accomplishing the anti-elimination of water. The replacement of the leaving group by solvent-derived hydrogen is stereorandom Bradyrhizobium japonicum oxaloacetate + H2O
-
 ?

Synonyms

Synonyms Comment Organism
bll6730
-
 Bradyrhizobium japonicum
TarD
-
 Bradyrhizobium japonicum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0019
-
 (S,S)-Tartrate 25°C, pH 7.5, mutant enzyme H322Q Bradyrhizobium japonicum
0.0027
-
 (S,S)-Tartrate 25°C, pH 7.5, mutant enzyme H322A Bradyrhizobium japonicum
0.0058
-
 (S,S)-Tartrate 25°C, pH 7.5, mutant enzyme K184R Bradyrhizobium japonicum
0.0069
-
 (S,S)-Tartrate 25°C, pH 7.5, mutant enzyme H322N Bradyrhizobium japonicum
7.3
-
 (S,S)-Tartrate 25°C, pH 7.5, wild-type enzyme Bradyrhizobium japonicum