Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 4.2.1.81 extracted from

  • Rode, H.; Giffhorn, F.
    D-(-)-tartrate dehydratase of Rhodopseudomonas sphaeroides: purification, characterization, and application to enzymatic determination of D-(-)-tartrate (1982), J. Bacteriol., 150, 1061-1068.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.2.1.81

Crystallization (Commentary)

Crystallization (Comment) Organism
-
 Cereibacter sphaeroides

General Stability

General Stability Organism
omission of Mg2+ from standard buffer results in rapid inactivation during purification with irreversible loss of activity after ammonium sulfate precipitation, Mg2+, Mn2+, Zn2+, Ca2+ or Co2+ stabilizes, not Ni2+ Cereibacter sphaeroides

Inhibitors

Inhibitors Comment Organism Structure
beta-mercaptoethanol
-
 Cereibacter sphaeroides
citrate weak Cereibacter sphaeroides
Cu2+
-
 Cereibacter sphaeroides
D-malate weak Cereibacter sphaeroides
dithioerythritol
-
 Cereibacter sphaeroides
EDTA strong Cereibacter sphaeroides
Fe2+
-
 Cereibacter sphaeroides
glycolate weak Cereibacter sphaeroides
L(+)-Tartrate weak Cereibacter sphaeroides
L-malate weak Cereibacter sphaeroides
meso-tartrate
-
 Cereibacter sphaeroides
additional information no inhibition by iodoacetamide, N-ethylmaleimide; no inhibition by iodoacetate Cereibacter sphaeroides

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.18
-
 (S,S)-Tartrate
-
 Cereibacter sphaeroides

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
 Cereibacter sphaeroides 5829
-

Metals/Ions

Metals/Ions Comment Organism Structure
divalent cations not Cereibacter sphaeroides

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
39500
-
 4 * 39500, SDS-PAGE Cereibacter sphaeroides
158000
-
 gel filtration, ultracentrifugation Cereibacter sphaeroides

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(S,S)-tartrate Cereibacter sphaeroides
-
 oxaloacetate + H2O
-
 ir
(S,S)-tartrate Cereibacter sphaeroides Si4 / DSM 8371
-
 oxaloacetate + H2O
-
 ir
additional information Cereibacter sphaeroides involved in D-tartrate catabolism ?
-
 ?
additional information Cereibacter sphaeroides Si4 / DSM 8371 involved in D-tartrate catabolism ?
-
 ?

Organism

Organism UniProt Comment Textmining
Cereibacter sphaeroides
-
-
-
Cereibacter sphaeroides Si4 / DSM 8371
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Cereibacter sphaeroides

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
7.2
-
-
 Cereibacter sphaeroides

Storage Stability

Storage Stability Organism
-20°C, 1.5 mg enzyme/ml 0.1 M Tris-HCl buffer, pH 7.2, 1 mM MgCl2, 20% loss of activity within 5 months Cereibacter sphaeroides
22°C, 50% loss of activity within 10 days Cereibacter sphaeroides
4°C, 50% loss of activity within 6 weeks Cereibacter sphaeroides

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S,S)-tartrate
-
 Cereibacter sphaeroides oxaloacetate + H2O
-
 ir
(S,S)-tartrate no substrates are L(+)-tartrate, meso-tartrate, glyceraldehyde, glycerate, glycerol, gluconate, 6-phosphogluconate, galactonate, mannonate, mannitol or sorbitol Cereibacter sphaeroides oxaloacetate + H2O at high or low pH-values or in the presence of metal ions spontaneous decarboxylation to pyruvate ir
(S,S)-tartrate
-
 Cereibacter sphaeroides Si4 / DSM 8371 oxaloacetate + H2O
-
 ir
(S,S)-tartrate no substrates are L(+)-tartrate, meso-tartrate, glyceraldehyde, glycerate, glycerol, gluconate, 6-phosphogluconate, galactonate, mannonate, mannitol or sorbitol Cereibacter sphaeroides Si4 / DSM 8371 oxaloacetate + H2O at high or low pH-values or in the presence of metal ions spontaneous decarboxylation to pyruvate ir
additional information involved in D-tartrate catabolism Cereibacter sphaeroides ?
-
 ?
additional information involved in D-tartrate catabolism Cereibacter sphaeroides Si4 / DSM 8371 ?
-
 ?

Subunits

Subunits Comment Organism
tetramer 4 * 39500, SDS-PAGE Cereibacter sphaeroides

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Cereibacter sphaeroides
48
-
-
 Cereibacter sphaeroides

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30
-
 stable at least 5 min at pH 6-8.7, 50% loss of activity at pH 4.9 and pH 9.4 after 5 min Cereibacter sphaeroides
45
-
 30 min, 50% loss of activity Cereibacter sphaeroides
50
-
 3 min, 50% loss of activity Cereibacter sphaeroides
55
-
 1 min, 50% loss of activity Cereibacter sphaeroides
60
-
 12 s, 50% loss of activity Cereibacter sphaeroides

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
19.8
-
 D-Tartrate
-
 Cereibacter sphaeroides

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
additional information
-
 highest activity in Tris-HCl buffer, less effective are N-2'-hydroxyethylpiperazine-2-ethanesulfonic acid, glycylglycine, triethanolamine-HCl, N-tris(hydroxymethyl)methyl-2-aminoethanesulfonic acid, imidazole or potassium phosphate buffer Cereibacter sphaeroides
6.2 7.2
-
 Cereibacter sphaeroides

pH Stability

pH Stability pH Stability Maximum Comment Organism
4.9
-
 5 min, 50% loss of activity at 30°C Cereibacter sphaeroides
6 8.7 stable at least 5 min at 30°C Cereibacter sphaeroides
9.4
-
 5 min, 50% loss of activity at 30°C Cereibacter sphaeroides

pI Value

Organism Comment pI Value Maximum pI Value
Cereibacter sphaeroides isoelectric focusing
-
 5.5