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Literature summary for 4.2.1.80 extracted from

  • Harayama, S.; Rekik, M.; Ngai, K.L.; Ornston, L.N.
    Physically associated enzymes produce and metabolize 2-hydroxy-2,4-dienoate, a chemically unstable intermediate in catechol metabolism via meta cleavage in Pseudomonas putida (1989), J. Bacteriol., 171, 6221-6228.
No PubMed abstract available

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Pseudomonas putida

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.03
-
4-hydroxy-2-oxopentanoate pH 7, native enzyme Pseudomonas putida

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ can replace Mn2+ Pseudomonas putida
Mn2+
-
Pseudomonas putida

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
28000
-
gel filtration Pseudomonas putida

Organism

Organism UniProt Comment Textmining
Pseudomonas putida
-
TOL-plasmid encoded
-
Pseudomonas putida
-
TOL plasmid-haboring
-
Pseudomonas putida mt-2 / ATCC 33015 / DSM 3931 / NCIB 12182 / NCIMB 12182
-
TOL plasmid-haboring
-

Purification (Commentary)

Purification (Comment) Organism
copurified with 4-oxalocrotonate decarboxylase Pseudomonas putida

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4
-
recombinant enzyme Pseudomonas putida
45
-
native enzyme Pseudomonas putida

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-hydroxy-2-oxopentanoate
-
Pseudomonas putida 2-oxopent-4-enoate + H2O
-
?
4-hydroxy-2-oxopentanoate
-
Pseudomonas putida mt-2 / ATCC 33015 / DSM 3931 / NCIB 12182 / NCIMB 12182 2-oxopent-4-enoate + H2O
-
?

Subunits

Subunits Comment Organism
More physically associated with 4-oxalocrotonate decarboxylase Pseudomonas putida

pH Range

pH Minimum pH Maximum Comment Organism
5.8 7.5
-
Pseudomonas putida