Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Pseudomonas putida |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.03 | - |
4-hydroxy-2-oxopentanoate | pH 7, native enzyme | Pseudomonas putida |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | can replace Mn2+ | Pseudomonas putida | |
Mn2+ | - |
Pseudomonas putida |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
28000 | - |
gel filtration | Pseudomonas putida |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas putida | - |
TOL-plasmid encoded | - |
Pseudomonas putida | - |
TOL plasmid-haboring | - |
Pseudomonas putida mt-2 / ATCC 33015 / DSM 3931 / NCIB 12182 / NCIMB 12182 | - |
TOL plasmid-haboring | - |
Purification (Comment) | Organism |
---|---|
copurified with 4-oxalocrotonate decarboxylase | Pseudomonas putida |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
4 | - |
recombinant enzyme | Pseudomonas putida |
45 | - |
native enzyme | Pseudomonas putida |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-hydroxy-2-oxopentanoate | - |
Pseudomonas putida | 2-oxopent-4-enoate + H2O | - |
? | |
4-hydroxy-2-oxopentanoate | - |
Pseudomonas putida mt-2 / ATCC 33015 / DSM 3931 / NCIB 12182 / NCIMB 12182 | 2-oxopent-4-enoate + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | physically associated with 4-oxalocrotonate decarboxylase | Pseudomonas putida |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.8 | 7.5 | - |
Pseudomonas putida |