Activating Compound | Comment | Organism | Structure |
---|---|---|---|
propionate | induces the enzyme expression from acnC and prpD | Escherichia coli |
Cloned (Comment) | Organism |
---|---|
DNA and amino acid sequence determination and analysis, genomic organization, expression of AcnC in AcnABnull strain, overexpression of PrpD in AcnABnull strain | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | enzyme possesses 1 unstable iron-sulfur center per monomer, required for activity, can be reconstituted by Fe2+ under reducing conditions | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
54000 | - |
gel filtration and native PAGE | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-Hydroxybutane-1,2,3-tricarboxylate | Escherichia coli | metabolic function | (Z)-But-2-ene-1,2,3-tricarboxylate + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
K12, several strains, gene prpD of the propionate catabolism operon prpRBCDE, the enzyme is identical with aconitase AcnC | - |
Purification (Comment) | Organism |
---|---|
AcnC partially 0.25fold from AcnABnull strain, PrpD 14.3fold from overexpression in AcnABnull strain | Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.005 | - |
partially purified AcnC | Escherichia coli |
0.8 | - |
purified PrpD | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-malate | - |
Escherichia coli | ? | - |
? | |
2-Hydroxybutane-1,2,3-tricarboxylate | i.e. 2-methylcitrate, best substrate | Escherichia coli | (Z)-But-2-ene-1,2,3-tricarboxylate + H2O | i.e. 2-methyl-cis-aconitate | ? | |
2-Hydroxybutane-1,2,3-tricarboxylate | metabolic function | Escherichia coli | (Z)-But-2-ene-1,2,3-tricarboxylate + H2O | - |
? | |
citrate | - |
Escherichia coli | ? | - |
? | |
D-malate | - |
Escherichia coli | ? | - |
? | |
D-tartrate | - |
Escherichia coli | ? | - |
? | |
DL-citramalate | - |
Escherichia coli | ? | - |
? | |
DL-isocitrate | - |
Escherichia coli | ? | - |
? | |
L-tartrate | - |
Escherichia coli | ? | - |
? | |
meso-tartrate | - |
Escherichia coli | ? | - |
? | |
additional information | substrate specificity, no activity with cis-aconitate and related substances containing double bonds, overview | Escherichia coli | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 54000, SDS-PAGE | Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
PrpD | - |
Escherichia coli |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Escherichia coli |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
half-life is 30 min for both AcnC and PrpD | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | 8 | for AcnC and PrpD | Escherichia coli |