Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 4.2.1.79 extracted from

  • Blank, L.; Green, J.; Guest, J.R.
    AcnC of Escherichia coli is a 2-methylcitrate dehydratase (PrpD) that can use citrate and isocitrate as substrates (2002), Microbiology, 148, 133-146.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
propionate induces the enzyme expression from acnC and prpD Escherichia coli

Cloned(Commentary)

Cloned (Comment) Organism
DNA and amino acid sequence determination and analysis, genomic organization, expression of AcnC in AcnABnull strain, overexpression of PrpD in AcnABnull strain Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ enzyme possesses 1 unstable iron-sulfur center per monomer, required for activity, can be reconstituted by Fe2+ under reducing conditions Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
54000
-
gel filtration and native PAGE Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2-Hydroxybutane-1,2,3-tricarboxylate Escherichia coli metabolic function (Z)-But-2-ene-1,2,3-tricarboxylate + H2O
-
?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
K12, several strains, gene prpD of the propionate catabolism operon prpRBCDE, the enzyme is identical with aconitase AcnC
-

Purification (Commentary)

Purification (Comment) Organism
AcnC partially 0.25fold from AcnABnull strain, PrpD 14.3fold from overexpression in AcnABnull strain Escherichia coli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.005
-
partially purified AcnC Escherichia coli
0.8
-
purified PrpD Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-malate
-
Escherichia coli ?
-
?
2-Hydroxybutane-1,2,3-tricarboxylate i.e. 2-methylcitrate, best substrate Escherichia coli (Z)-But-2-ene-1,2,3-tricarboxylate + H2O i.e. 2-methyl-cis-aconitate ?
2-Hydroxybutane-1,2,3-tricarboxylate metabolic function Escherichia coli (Z)-But-2-ene-1,2,3-tricarboxylate + H2O
-
?
citrate
-
Escherichia coli ?
-
?
D-malate
-
Escherichia coli ?
-
?
D-tartrate
-
Escherichia coli ?
-
?
DL-citramalate
-
Escherichia coli ?
-
?
DL-isocitrate
-
Escherichia coli ?
-
?
L-tartrate
-
Escherichia coli ?
-
?
meso-tartrate
-
Escherichia coli ?
-
?
additional information substrate specificity, no activity with cis-aconitate and related substances containing double bonds, overview Escherichia coli ?
-
?

Subunits

Subunits Comment Organism
monomer 1 * 54000, SDS-PAGE Escherichia coli

Synonyms

Synonyms Comment Organism
PrpD
-
Escherichia coli

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Escherichia coli

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
half-life is 30 min for both AcnC and PrpD Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7 8 for AcnC and PrpD Escherichia coli