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Literature summary for 4.2.1.76 extracted from

  • Martinez, V.; Ingwers, M.; Smith, J.; Glushka, J.; Yang, T.; Bar-Peled, M.
    Biosynthesis of UDP-4-keto-6-deoxyglucose and UDP-rhamnose in pathogenic fungi Magnaporthe grisea and Botryotinia fuckeliana (2012), J. Biol. Chem., 287, 879-892.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21 cells Botrytis cinerea
expressed in Escherichia coli BL21 cells Pyricularia grisea

Inhibitors

Inhibitors Comment Organism Structure
additional information not inhibited by NADH Botrytis cinerea
additional information not inhibited by NADH Pyricularia grisea

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
52000
-
3 * 52000, SDS-PAGE Pyricularia grisea
52500
-
3 * 52500, SDS-PAGE Botrytis cinerea
140000
-
gel filtration Botrytis cinerea
140000
-
gel filtration Pyricularia grisea

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
UDP-glucose Botrytis cinerea
-
UDP-4-dehydro-6-deoxy-D-glucose + H2O
-
?
UDP-glucose Pyricularia grisea
-
UDP-4-dehydro-6-deoxy-D-glucose + H2O
-
?
UDP-glucose Pyricularia grisea CP987
-
UDP-4-dehydro-6-deoxy-D-glucose + H2O
-
?
UDP-glucose Botrytis cinerea B05.10
-
UDP-4-dehydro-6-deoxy-D-glucose + H2O
-
?

Organism

Organism UniProt Comment Textmining
Botrytis cinerea F8U971
-
-
Botrytis cinerea B05.10 F8U971
-
-
Pyricularia grisea F8U969
-
-
Pyricularia grisea CP987 F8U969
-
-

Purification (Commentary)

Purification (Comment) Organism
nickel-Sepharose column chromatography Botrytis cinerea
nickel-Sepharose column chromatography Pyricularia grisea

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dTDP-glucose this enzymatic reaction is much less efficient compared to UDP-glucose Botrytis cinerea dTDP-4-dehydro-6-deoxy-D-glucose + H2O
-
?
dTDP-glucose this enzymatic reaction is much less efficient compared to UDP-glucose Pyricularia grisea dTDP-4-dehydro-6-deoxy-D-glucose + H2O
-
?
dTDP-glucose this enzymatic reaction is much less efficient compared to UDP-glucose Pyricularia grisea CP987 dTDP-4-dehydro-6-deoxy-D-glucose + H2O
-
?
dTDP-glucose this enzymatic reaction is much less efficient compared to UDP-glucose Botrytis cinerea B05.10 dTDP-4-dehydro-6-deoxy-D-glucose + H2O
-
?
UDP-glucose
-
Botrytis cinerea UDP-4-dehydro-6-deoxy-D-glucose + H2O
-
?
UDP-glucose
-
Pyricularia grisea UDP-4-dehydro-6-deoxy-D-glucose + H2O
-
?
UDP-glucose
-
Pyricularia grisea CP987 UDP-4-dehydro-6-deoxy-D-glucose + H2O
-
?
UDP-glucose
-
Botrytis cinerea B05.10 UDP-4-dehydro-6-deoxy-D-glucose + H2O
-
?

Subunits

Subunits Comment Organism
homotrimer 3 * 52500, SDS-PAGE Botrytis cinerea
homotrimer 3 * 52000, SDS-PAGE Pyricularia grisea

Synonyms

Synonyms Comment Organism
UDP-glucose-4,6-dehydratase
-
Botrytis cinerea
UDP-glucose-4,6-dehydratase
-
Pyricularia grisea
UG4,6-Dh
-
Botrytis cinerea
UG4,6-Dh
-
Pyricularia grisea

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25 30
-
Botrytis cinerea
25 30
-
Pyricularia grisea

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
Botrytis cinerea
7
-
-
Pyricularia grisea

Cofactor

Cofactor Comment Organism Structure
NAD+ required Botrytis cinerea
NAD+ required Pyricularia grisea