Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21 cells | Botrytis cinerea |
expressed in Escherichia coli BL21 cells | Pyricularia grisea |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | not inhibited by NADH | Botrytis cinerea | |
additional information | not inhibited by NADH | Pyricularia grisea |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
52000 | - |
3 * 52000, SDS-PAGE | Pyricularia grisea |
52500 | - |
3 * 52500, SDS-PAGE | Botrytis cinerea |
140000 | - |
gel filtration | Botrytis cinerea |
140000 | - |
gel filtration | Pyricularia grisea |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-glucose | Botrytis cinerea | - |
UDP-4-dehydro-6-deoxy-D-glucose + H2O | - |
? | |
UDP-glucose | Pyricularia grisea | - |
UDP-4-dehydro-6-deoxy-D-glucose + H2O | - |
? | |
UDP-glucose | Pyricularia grisea CP987 | - |
UDP-4-dehydro-6-deoxy-D-glucose + H2O | - |
? | |
UDP-glucose | Botrytis cinerea B05.10 | - |
UDP-4-dehydro-6-deoxy-D-glucose + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Botrytis cinerea | F8U971 | - |
- |
Botrytis cinerea B05.10 | F8U971 | - |
- |
Pyricularia grisea | F8U969 | - |
- |
Pyricularia grisea CP987 | F8U969 | - |
- |
Purification (Comment) | Organism |
---|---|
nickel-Sepharose column chromatography | Botrytis cinerea |
nickel-Sepharose column chromatography | Pyricularia grisea |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dTDP-glucose | this enzymatic reaction is much less efficient compared to UDP-glucose | Botrytis cinerea | dTDP-4-dehydro-6-deoxy-D-glucose + H2O | - |
? | |
dTDP-glucose | this enzymatic reaction is much less efficient compared to UDP-glucose | Pyricularia grisea | dTDP-4-dehydro-6-deoxy-D-glucose + H2O | - |
? | |
dTDP-glucose | this enzymatic reaction is much less efficient compared to UDP-glucose | Pyricularia grisea CP987 | dTDP-4-dehydro-6-deoxy-D-glucose + H2O | - |
? | |
dTDP-glucose | this enzymatic reaction is much less efficient compared to UDP-glucose | Botrytis cinerea B05.10 | dTDP-4-dehydro-6-deoxy-D-glucose + H2O | - |
? | |
UDP-glucose | - |
Botrytis cinerea | UDP-4-dehydro-6-deoxy-D-glucose + H2O | - |
? | |
UDP-glucose | - |
Pyricularia grisea | UDP-4-dehydro-6-deoxy-D-glucose + H2O | - |
? | |
UDP-glucose | - |
Pyricularia grisea CP987 | UDP-4-dehydro-6-deoxy-D-glucose + H2O | - |
? | |
UDP-glucose | - |
Botrytis cinerea B05.10 | UDP-4-dehydro-6-deoxy-D-glucose + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
homotrimer | 3 * 52500, SDS-PAGE | Botrytis cinerea |
homotrimer | 3 * 52000, SDS-PAGE | Pyricularia grisea |
Synonyms | Comment | Organism |
---|---|---|
UDP-glucose-4,6-dehydratase | - |
Botrytis cinerea |
UDP-glucose-4,6-dehydratase | - |
Pyricularia grisea |
UG4,6-Dh | - |
Botrytis cinerea |
UG4,6-Dh | - |
Pyricularia grisea |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 30 | - |
Botrytis cinerea |
25 | 30 | - |
Pyricularia grisea |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Botrytis cinerea |
7 | - |
- |
Pyricularia grisea |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | required | Botrytis cinerea | |
NAD+ | required | Pyricularia grisea |