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Literature summary for 4.2.1.75 extracted from
- Purification and properties of uroporphyrinogen III synthase (co-synthase) from an overproducing recombinant strain of Escherichia coli K-12 (1989), Biochem. J., 264, 397-402.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| overproduction | Escherichia coli |
General Stability
| General Stability | Organism |
|---|---|
| purification needs to be carried out as close to 0¦C as possible | Escherichia coli |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 5,5'-dithiobis(2-nitrobenzoate) | - |
Escherichia coli |  |
| diethyl dicarbonate | - |
Escherichia coli | |
| iodoacetamide | - |
Escherichia coli | |
| iodoacetic acid | - |
Escherichia coli | |
| N-ethylmaleimide | - |
Escherichia coli | |
| p-chloromercuribenzoate | - |
Escherichia coli | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.005 | - |
Hydroxymethylbilane | - |
Escherichia coli | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| additional information | bivalent ions fail to activate or stabilize the enzyme significantly | Escherichia coli |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 26000 | - |
SDS-PAGE | Escherichia coli |
| 29000 | - |
gel filtration | Escherichia coli |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| from overproducing strain | Escherichia coli |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 25 | - |
hydroxymethylbilane | Escherichia coli |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20¦C, stable to freezing | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Hydroxymethylbilane | - |
Escherichia coli | Uroporphyrinogen III | - |
? | |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | - |
20% loss of activity in 10 min | Escherichia coli |
| 60 | - |
completely inactivated in 1 min | Escherichia coli |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 500 | - |
Hydroxymethylbilane | - |
Escherichia coli | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.8 | - |
- |
Escherichia coli |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 9 | at pH 7.0: 60% of optimum activity, at pH 9.0: 60% of optimum activity | Escherichia coli |
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