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Literature summary for 4.2.1.74 extracted from

  • Luo, M.J.; He, X.Y.; Sprecher, H.; Schulz, H.
    Purification and characterization of the trifunctional beta-oxidation complex from pig heart mitochondria (1993), Arch. Biochem. Biophys., 304, 266-271.
    View publication on PubMed

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion inner membrane Sus scrofa 5739
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
45000
-
x * 81000 + x * 45000, SDS-PAGE Sus scrofa
81000
-
x * 81000 + x * 45000, SDS-PAGE Sus scrofa

Organism

Organism UniProt Comment Textmining
Sus scrofa
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Sus scrofa

Source Tissue

Source Tissue Comment Organism Textmining
heart
-
Sus scrofa
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information activity of the trifunctional enzyme complex with C4 substrates is less than 2% of the long-chain activities. The trifunctional beta-oxidation complex contains long-chain specific activities of enoyl-CoA hydratase, L-3-hydroxyacyl-CoA dehydrogenase, and 3-ketoacyl-CoA thiolase Sus scrofa ?
-
?
trans-2-decenoyl-CoA + H2O 86% of the activity with trans-2-tetradecenoyl-CoA Sus scrofa (3S)-3-hydroxydecanoyl-CoA
-
?
trans-2-hexadecenoyl-CoA + H2O 50% of the activity with trans-2-tetradecenoyl-CoA Sus scrofa (3S)-3-hydroxyhexadecanoyl-CoA
-
?
trans-2-hexenoyl-CoA + H2O 57% of the activity with trans-2-tetradecenoyl-CoA Sus scrofa (3S)-3-hydroxyhexanoyl-CoA
-
?
trans-2-octenoyl-CoA + H2O 71% of the activity with trans-2-tetradecenoyl-CoA Sus scrofa (3S)-3-hydroxyoctanoyl-CoA
-
?
trans-2-tetradecenoyl-CoA + H2O best substrate Sus scrofa (3S)-3-hydroxytetradecanoyl-CoA
-
?

Subunits

Subunits Comment Organism
? x * 81000 + x * 45000, SDS-PAGE Sus scrofa

Synonyms

Synonyms Comment Organism
enoyl-CoA hydratase a component enzyme of the trifunctional beta-oxidation complex, which is associated with the inner membrane of the mitochondria, contains long-chain specific activities of enoyl-CoA hydratase, L-3-hydroxyacyl-CoA dehydrogenase, and 3-ketoacyl-CoA thiolase Sus scrofa