Literature summary for 4.2.1.69 extracted from

Kirubakaran, S.I.; Sakthivel, N.
Site-directed mutagenesis, heterologous expression of cyanamide hydratase gene and antimicrobial activity of cyanamide (2008), Curr. Microbiol., 56, 42-47.

Application

Application	Comment	Organism
agriculture	due to its innate ability to convert cyanamide to urea and the broad-spectrum antimicrobial activity of cyanamide, the cah gene can be used to facilitate plant growth promotion and biocontrol of phytopathogens	Albifimbria verrucaria

Cloned(Commentary)

Cloned (Comment)	Organism
wild-type cah and its mutants are cloned and overexpressed in pQE-60 Escherichia coli expression system. Western blot analysis confirms the production of 27700 Da cah enzyme by all the recombinants. Agrobacterium-mediated transformation is performed in Nicotiana tabaccum cv. Samsun plants by employing the leaf-disc method. The integration and expression of cah gene in transgenic plants are confirmed by polymerase chain reaction, Southern and Western blot analyses	Albifimbria verrucaria

Cloned (Comment)

Organism

wild-type cah and its mutants are cloned and overexpressed in pQE-60 Escherichia coli expression system. Western blot analysis confirms the production of 27700 Da cah enzyme by all the recombinants. Agrobacterium-mediated transformation is performed in Nicotiana tabaccum cv. Samsun plants by employing the leaf-disc method. The integration and expression of cah gene in transgenic plants are confirmed by polymerase chain reaction, Southern and Western blot analyses

Albifimbria verrucaria

Organism

Organism	UniProt	Comment	Textmining
Albifimbria verrucaria	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Albifimbria verrucaria

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Release 2023.1 (January 2023)