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Literature summary for 4.2.1.66 extracted from
- Genome mining of cyanide-degrading nitrilases from filamentous fungi (2008), Appl. Microbiol. Biotechnol., 80, 427-435.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| cyanide | expression induced in presence of | Aspergillus nidulans |  |
| cyanide | expression induced in presence of | Neurospora crassa | |
Application
| Application | Comment | Organism |
|---|---|---|
| biotechnology | searching for enzymes in the bioremediation of cyanide-containing waste | Aspergillus nidulans |
| biotechnology | searching for enzymes in the bioremediation of cyanide-containing waste | Neurospora crassa |
| biotechnology | searching for enzymes in the bioremediation of cyanide-containing waste | Fusarium graminearum |
| biotechnology | searching for enzymes in the bioremediation of cyanide-containing waste | Microdochium sorghi |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli, strains and plasmids listed | Aspergillus nidulans |
| expressed in Escherichia coli, strains and plasmids listed | Neurospora crassa |
| expressed in Escherichia coli, strains and plasmids listed | Fusarium graminearum |
| expressed in Escherichia coli, strains and plasmids listed | Microdochium sorghi |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| additional information | rate of the reaction decreases at high substrate concentrations, indicative of substrate inhibition | Aspergillus nidulans | |
| additional information | rate of the reaction decreases at high substrate concentrations, indicative of substrate inhibition | Neurospora crassa |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| HCN + H2O | Aspergillus nidulans | - |
formamide | - |
? | |
| HCN + H2O | Neurospora crassa | - |
formamide | - |
? | |
| HCN + H2O | Fusarium graminearum | - |
formamide | - |
? | |
| HCN + H2O | Microdochium sorghi | - |
formamide | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aspergillus nidulans | - |
- |
- |
| Fusarium graminearum | - |
- |
- |
| Microdochium sorghi | P32964 | - |
- |
| Neurospora crassa | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant protein, gel filtration | Aspergillus nidulans |
| recombinant protein, gel filtration | Neurospora crassa |
| recombinant protein, gel filtration | Fusarium graminearum |
| recombinant protein, gel filtration | Microdochium sorghi |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
comparison of fungal nitrilases to determine best candidates for the remediation of cyanide-containing industrial wastewater, analysis according to relative specific activity, pH activity profile, thermal stability, and ability to remediate cyanide contaminated waste water from silver and copper electroplating baths | Fusarium graminearum |
| additional information | - |
comparison of fungal nitrilases to determine best candidates for the remediation of cyanide-containing industrial wastewater, enzyme of Aspergillus nidulans indicates highest reaction rate of the four fungal nitrilases compared in the study | Aspergillus nidulans |
| additional information | - |
comparison of fungal nitrilases to determine best candidates for the remediation of cyanide-containing industrial wastewater, lowest activity of the group of nitrilases compared in this study, does not reach maximum activity until 80 mM KCN | Microdochium sorghi |
| additional information | - |
comparison of fungal nitrilases to determine best candidates for the remediation of cyanide-containing industrial wastewater, most promising candidate isolated from Neurospora crassa, according to high activity over a wide range of pH values, stability for at least 48 h when incubated at temperatures ranging from 27°C to 43°C, highest rates at a substrate concentration of 20 mM KCN, maximal rates around 60 mM KCN | Neurospora crassa |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| HCN + H2O | - |
Aspergillus nidulans | formamide | - |
? | |
| HCN + H2O | - |
Neurospora crassa | formamide | - |
? | |
| HCN + H2O | - |
Fusarium graminearum | formamide | - |
? | |
| HCN + H2O | - |
Microdochium sorghi | formamide | - |
? | |
| HCN + H2O | emediation of cyanide-containing industrial wastewater analyzed | Aspergillus nidulans | formamide | - |
? | |
| HCN + H2O | emediation of cyanide-containing industrial wastewater analyzed | Neurospora crassa | formamide | - |
? | |
| HCN + H2O | emediation of cyanide-containing industrial wastewater analyzed | Fusarium graminearum | formamide | - |
? | |
| HCN + H2O | emediation of cyanide-containing industrial wastewater analyzed | Microdochium sorghi | formamide | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| CHT | - |
Aspergillus nidulans |
| CHT | - |
Neurospora crassa |
| CHT | - |
Fusarium graminearum |
| CHT | - |
Microdochium sorghi |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 27 | 50 | incubated at 27°C, 37°C, 43°C, and 50°C, activity measured at time points ranging between 0 and 48 h, at 37°C and 43°C most stable with no loss of activity after 48 h at 37°C, about 40% of activity remaining after 48 h at 43°C, at 50°C activity below 5% by 48 h | Neurospora crassa |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 27 | 50 | incubated at 27°C, 37°C, 43°C, and 50°C, activity measured at time points ranging between 0 and 48 h, CHT of Gibberella zeae shows an initial increase in activity at lower temperatures, subsequent stability is similar to that of Neurospora crassa, gradual decrease in activity at 50°C | Fusarium graminearum |
| 27 | 50 | incubated at 27°C, 37°C, 43°C, and 50°C, activity measured at time points ranging between 0 and 48 h, enzyme of Aspergillus nidulans quickly looses activity at the two highest temperatures, and 50% of activity is lost after 48 h at 37°C | Aspergillus nidulans |
| 27 | 50 | incubated at 27°C, 37°C, 43°C, and 50°C, activity measured at time points ranging between 0 and 48 h, enzyme quickly looses activity at the two highest temperatures, 50% of activity is lost after 48 h at 37°C | Microdochium sorghi |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5.2 | 9 | at least half-maximal activity between pH 5.2 and pH 9, widest range of activity in comparison to four other enzymes used in this study | Neurospora crassa |
| 5.2 | 9 | relatively wide range of >50% activity relative to its maximal rate | Aspergillus nidulans |
| 6 | 8.5 | at least half-maximal activity between approximately pH 6 and pH 8.5, narrower range of activity in comparison to four other enzymes used in this study | Fusarium graminearum |
| 6 | 8.5 | narrow range of activity in comparison to four other enzymes used in this study, more tolerant of higher than of lower pH values | Microdochium sorghi |
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