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Literature summary for 4.2.1.6 extracted from

  • Motter, F.A.; Kuivanen, J.; Keraenen, H.; Hilditch, S.; Penttilae, M.; Richard, P.
    Categorisation of sugar acid dehydratases in Aspergillus niger (2014), Fungal Genet. Biol., 64, 67-72 .
    View publication on PubMed
Search for more literature for 4.2.1.6

Cloned(Commentary)

Cloned (Comment) Organism
gene dgdA, DNA and amino acid sequence determination and analysis, recombinant expression in Saccharomyces cerevisiae Aspergillus niger
gene dgdB, DNA and amino acid sequence determination and analysis, recombinant expression in Saccharomyces cerevisiae Aspergillus niger

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information Michaelis-Menten kinetics Aspergillus niger
8.9
-
 D-galactonate recombinant enzyme, pH 7.0, temperature not specified in the publication Aspergillus niger
21.74
-
 D-galactonate recombinant enzyme, pH 7.0, temperature not specified in the publication Aspergillus niger

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-galactonate Aspergillus niger
-
 2-dehydro-3-deoxy-D-galactonate + H2O
-
 ?
D-galactonate Aspergillus niger ATCC 1015 / CBS 113.46 / FGSC A1144 / LSHB Ac4 / NCTC 3858a / NRRL 328 / USDA 3528.7
-
 2-dehydro-3-deoxy-D-galactonate + H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Aspergillus niger G3Y8T6
-
-
Aspergillus niger G3YE52
-
-
Aspergillus niger ATCC 1015 / CBS 113.46 / FGSC A1144 / LSHB Ac4 / NCTC 3858a / NRRL 328 / USDA 3528.7 G3Y8T6
-
-
Aspergillus niger ATCC 1015 / CBS 113.46 / FGSC A1144 / LSHB Ac4 / NCTC 3858a / NRRL 328 / USDA 3528.7 G3YE52
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-galactonate
-
 Aspergillus niger 2-dehydro-3-deoxy-D-galactonate + H2O
-
 ?
D-galactonate
-
 Aspergillus niger ATCC 1015 / CBS 113.46 / FGSC A1144 / LSHB Ac4 / NCTC 3858a / NRRL 328 / USDA 3528.7 2-dehydro-3-deoxy-D-galactonate + H2O
-
 ?
additional information the enzyme only shows activity with D-galactonate. The production of 2-dehydro-3-deoxy sugar is measured with the thiobarbituric acid assay. Substrate specificity analysis, overview. No activity with L-rhamnonate, L-galactonate, D-gulonate, D-gluconate, L-gulonate, D-arabonate, D-lyxonate, L-lyxonate, D-xylonate, mucic acid, L-fuconate, L-mannonate, D-mannonate, D-riborate, L-arabonate, D-galacturonate, and D-glucuronate Aspergillus niger ?
-
 ?
additional information the enzyme only shows activity with D-galactonate. The production of 2-dehydro-3-deoxy sugar is measured with the thiobarbituric acid assay. Substrate specificity analysis, overview. No activity with L-rhamnonate, L-galactonate, D-gulonate, D-gluconate, L-gulonate, D-arabonate, D-lyxonate, L-lyxonate, D-xylonate, mucic acid, L-fuconate, L-mannonate, D-mannonate, D-riborate, L-arabonate, D-galacturonate, and D-glucuronate Aspergillus niger ATCC 1015 / CBS 113.46 / FGSC A1144 / LSHB Ac4 / NCTC 3858a / NRRL 328 / USDA 3528.7 ?
-
 ?

Synonyms

Synonyms Comment Organism
D-galactonate dehydratase
-
 Aspergillus niger
dgdA
-
 Aspergillus niger
dgdB
-
 Aspergillus niger

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 assay at Aspergillus niger