Literature summary for 4.2.1.6 extracted from

Donald, A.; Sibley, D.; Lyons, D.E.; Dahms, A.S.
D-Galactonate dehydrase. Purification and properties (1979), J. Biol. Chem., 254, 2132-2137.

Search for more literature for 4.2.1.6

General Stability

General Stability	Organism
insensitive to repeated freeze-thaw cycles	Pseudomonas sp.
stable to lyophilization in 0.05 M glycine/0.01 M sodium cacodylate, pH 8.8	Pseudomonas sp.

Inhibitors

Inhibitors	Comment	Organism	Structure
EDTA	-	Pseudomonas sp.
iodoacetamide	-	Pseudomonas sp.
PCMB	-	Pseudomonas sp.

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
1.14	-	D-galactonate	-	Pseudomonas sp.

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Co2+	divalent cation required, in the order of decreasing effectiveness: Mg2+, Mn2+, Co2+, Ni2+	Pseudomonas sp.
Mg2+	optimal concentration: 1 mM	Pseudomonas sp.
Mg2+	divalent cation required, in the order of decreasing effectiveness: Mg2+, Mn2+, Co2+, Ni2+	Pseudomonas sp.
Mn2+	divalent cation required, in the order of decreasing effectiveness: Mg2+, Mn2+, Co2+, Ni2+	Pseudomonas sp.
Ni2+	divalent cation required, in the order of decreasing effectiveness: Mg2+, Mn2+, Co2+, Ni2+	Pseudomonas sp.

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
57000	-	4 * 57000, SDS-PAGE	Pseudomonas sp.
240000	245000	gel filtration, sucrose density gradient centrifugation	Pseudomonas sp.

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
D-galactonate	Pseudomonas sp.	growth on D-galactonate, L-arabinose and D-fucose increases the enzyme activity over the level of cells grown in nutrient broth	?	-	?

Organism

Organism	UniProt	Comment	Textmining
Pseudomonas sp.	-	ATCC 27855	-

Purification (Commentary)

Purification (Comment)	Organism
-	Pseudomonas sp.

Reaction

Reaction	Comment	Organism	Reaction ID
D-galactonate = 2-dehydro-3-deoxy-D-galactonate + H2O	the immediate dehydration product is the enol which undergoes spontaneous ketonization	Pseudomonas sp.	a

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
38	-	-	Pseudomonas sp.

Storage Stability

Storage Stability	Organism
-20°C, 6 months, stable	Pseudomonas sp.

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
D-galactonate	growth on D-galactonate, L-arabinose and D-fucose increases the enzyme activity over the level of cells grown in nutrient broth	Pseudomonas sp.	?	-	?
D-galactonate	-	Pseudomonas sp.	2-dehydro-3-deoxy-D-galactonate + H2O	-	?

Subunits

Subunits	Comment	Organism
tetramer	4 * 57000, SDS-PAGE	Pseudomonas sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
additional information	-	Mg2+, dithiothreitol and D-galactonate increase the rate of thermal inactivation	Pseudomonas sp.
53	-	half-life: more than 1 h	Pseudomonas sp.
81	-	half-life: 10 min	Pseudomonas sp.
95	-	half-life: 3 min	Pseudomonas sp.

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.1	-	glycylglycine buffer	Pseudomonas sp.

pH Range

pH Minimum	pH Maximum	Comment	Organism
5.9	8	pH 5.9: about 40% of maximal activity, pH 8.0: about 25% of maximal activity	Pseudomonas sp.

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Release 2023.1 (January 2023)