BRENDA - Enzyme Database
show all sequences of 4.2.1.57

Untersuchungen über den bakteriellen Abbau von Isoprenoiden. IV. Reinigung und Eigenschaften der beta-Isohexenylglutaconyl-CoA-hydratase und beta-Hydroxy-beta-isohexenylglutaryl-CoA-lyase

Seubert, W.; Fass, E.; Biochem. Z. 341, 23-34 (1964)
No PubMed abstract available

Data extracted from this reference:

Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Mg2+
added in enzyme assay
Pseudomonas citronellolis
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
additional information
Pseudomonas citronellolis
involved in the isoprenoid degradation pathway
?
-
-
?
additional information
Pseudomonas citronellolis
dicarbonic-acid-CoA-derivatives (alpha,beta-unsaturated, beta-substituted, formed by carboxylation of dimethylacryl-, geranyl- and farnesyl-CoA)
?
-
-
?
Organism
Organism
UniProt
Commentary
Textmining
Pseudomonas citronellolis
-
-
-
Purification (Commentary)
Purification (Commentary)
Organism
-
Pseudomonas citronellolis
Reaction
Reaction
Commentary
Organism
Reaction ID
3-Hydroxy-3-(4-methylpent-3-en-1-yl)glutaryl-CoA = 3-(4-methylpent-3-en-1-yl)pent-2-enedioyl-CoA + H2O
mechanism
Pseudomonas citronellolis
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
25.8
-
-
Pseudomonas citronellolis
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
3-(4-methylpent-3-en-1-yl)pent-2-enedioyl-CoA
no literature for reverse raction i.e elimination
5337
Pseudomonas citronellolis
3-hydroxy-3-(4-methylpent-3-en-1-yl)glutaryl-CoA
hydroxyisohexenylglutaryl-CoA + H2O
5337
Pseudomonas citronellolis
?
3-(4-methylpent-3-en-1-yl)pent-2-enedioyl-CoA
isohexenylglutaconyl-CoA
5337
Pseudomonas citronellolis
3-hydroxy-3-(4-methylpent-3-en-1-yl)glutaryl-CoA
hydroxyisohexenylglutaryl-CoA + H2O
5337
Pseudomonas citronellolis
?
additional information
specifity for CoA-derivatives of beta-substituted, alpha, beta-unsaturated dicarbonic acids formed by carboxylation of dimetylacryl-, geranyl- and farnesyl-CoA
5337
Pseudomonas citronellolis
?
-
-
-
?
additional information
involved in the isoprenoid degradation pathway
5337
Pseudomonas citronellolis
?
-
-
-
?
additional information
dicarbonic-acid-CoA-derivatives (alpha,beta-unsaturated, beta-substituted, formed by carboxylation of dimethylacryl-, geranyl- and farnesyl-CoA)
5337
Pseudomonas citronellolis
?
-
-
-
?
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
22
-
enzyme assay at
Pseudomonas citronellolis
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.7
-
,enzyme assay at
Pseudomonas citronellolis
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Mg2+
added in enzyme assay
Pseudomonas citronellolis
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
additional information
Pseudomonas citronellolis
involved in the isoprenoid degradation pathway
?
-
-
?
additional information
Pseudomonas citronellolis
dicarbonic-acid-CoA-derivatives (alpha,beta-unsaturated, beta-substituted, formed by carboxylation of dimethylacryl-, geranyl- and farnesyl-CoA)
?
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
-
Pseudomonas citronellolis
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
25.8
-
-
Pseudomonas citronellolis
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
3-(4-methylpent-3-en-1-yl)pent-2-enedioyl-CoA
no literature for reverse raction i.e elimination
5337
Pseudomonas citronellolis
3-hydroxy-3-(4-methylpent-3-en-1-yl)glutaryl-CoA
hydroxyisohexenylglutaryl-CoA + H2O
5337
Pseudomonas citronellolis
?
3-(4-methylpent-3-en-1-yl)pent-2-enedioyl-CoA
isohexenylglutaconyl-CoA
5337
Pseudomonas citronellolis
3-hydroxy-3-(4-methylpent-3-en-1-yl)glutaryl-CoA
hydroxyisohexenylglutaryl-CoA + H2O
5337
Pseudomonas citronellolis
?
additional information
specifity for CoA-derivatives of beta-substituted, alpha, beta-unsaturated dicarbonic acids formed by carboxylation of dimetylacryl-, geranyl- and farnesyl-CoA
5337
Pseudomonas citronellolis
?
-
-
-
?
additional information
involved in the isoprenoid degradation pathway
5337
Pseudomonas citronellolis
?
-
-
-
?
additional information
dicarbonic-acid-CoA-derivatives (alpha,beta-unsaturated, beta-substituted, formed by carboxylation of dimethylacryl-, geranyl- and farnesyl-CoA)
5337
Pseudomonas citronellolis
?
-
-
-
?
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
22
-
enzyme assay at
Pseudomonas citronellolis
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.7
-
,enzyme assay at
Pseudomonas citronellolis
Other publictions for EC 4.2.1.57
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
746831
Poudel
The Pseudomonas aeruginosa is ...
Pseudomonas aeruginosa
Appl. Environ. Microbiol.
81
6558-6566
2015
-
-
1
1
-
-
-
-
-
-
-
-
-
6
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
1
1
-
-
5337
Seubert
-
Untersuchungen über den bakte ...
Pseudomonas citronellolis
Biochem. Z.
341
23-34
1964
-
-
-
-
-
-
-
-
-
1
-
2
-
1
-
-
1
1
-
-
1
-
5
-
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
2
-
-
-
1
-
-
1
-
5
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-