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Literature summary for 4.2.1.55 extracted from

  • Moskowitz, G.J.; Merrick, J.M.
    Metabolism of poly-beta-hydroxybutyrate. II. Enzymatic synthesis of D(-)-beta-hydroxybutyryl coenzyme A by an enoyl hydrase from Rhodospirillum rubrum (1969), Biochemistry, 8, 2748-2755.
    View publication on PubMed
Search for more literature for 4.2.1.55

Inhibitors

Inhibitors Comment Organism Structure
iodoacetamide 0.01 M, 30% inhibition Rhodospirillum rubrum
iodoacetic acid 0.01 M, 12% inhibition Rhodospirillum rubrum
N-ethylmaleimide 0.01 M, 23% inhibition Rhodospirillum rubrum
p-Substituted mercuribenzoate 3 mM, 65% inhibition Rhodospirillum rubrum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00926
-
 crotonyl-CoA
-
 Rhodospirillum rubrum
0.0263
-
 trans-2-hexenoyl-CoA
-
 Rhodospirillum rubrum
0.08
-
 crotonyl-[acyl-carrier protein]
-
 Rhodospirillum rubrum
0.118
-
 Crotonylpantetheine
-
 Rhodospirillum rubrum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
crotonyl-CoA Rhodospirillum rubrum enzyme is involved in synthesis of poly-beta-hydroxybutyrate, lipid reserve of bacteria ?
-
 ?

Organism

Organism UniProt Comment Textmining
Rhodospirillum rubrum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Rhodospirillum rubrum

Reaction

Reaction Comment Organism Reaction ID
(3R)-3-hydroxybutanoyl-CoA = crotonoyl-CoA + H2O stereochemistry Rhodospirillum rubrum
a

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Rhodospirillum rubrum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(3R)-3-hydroxybutanoyl-CoA
-
 Rhodospirillum rubrum crotonyl-CoA + H2O
-
 ?
crotonyl-CoA enzyme is involved in synthesis of poly-beta-hydroxybutyrate, lipid reserve of bacteria Rhodospirillum rubrum ?
-
 ?
Crotonyl-CoA + H2O
-
 Rhodospirillum rubrum (3R)-3-Hydroxybutanoyl-CoA
-
 ?
Crotonyl-[acyl-carrier protein] + H2O about 50% of the activity with crotonyl-CoA Rhodospirillum rubrum ?
-
 ?
Crotonylpantetheine about 55% of the activity with crotonyl-CoA Rhodospirillum rubrum ?
-
 ?
additional information no activity with crotonylglutathione, acrylyl-CoA Rhodospirillum rubrum ?
-
 ?
trans-2-Hexenoyl-CoA + H2O about 35% of the activity with crotonyl-CoA Rhodospirillum rubrum ?
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.4 8.6
-
 Rhodospirillum rubrum