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Literature summary for 4.2.1.47 extracted from

  • Bisso, A.; Sturla, L.; Zanardi, D.; De Flora, A.; Tonetti, M.
    Structural and enzymatic characterization of human recombinant GDP-D-mannose-4,6-dehydratase (1999), FEBS Lett., 456, 370-374.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
L-GMD, S-GMD and M-GMD, expressed in Escherichia coli Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
GDP competitive Homo sapiens
GDP-L-fucose non-competitive Homo sapiens
GDP-L-glucose competitive Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0035
-
GDP-mannose pH 8, isoenzyme M-GMD Homo sapiens
0.00364
-
GDP-mannose pH 8, isoenzyme L-GMD Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
38700
-
isoenzyme S-GMD, SDS-PAGE Homo sapiens
40200
-
isoenzyme M-GMD, SDS-PAGE Homo sapiens
42000
-
isoenzyme L-GMD, SDS-PAGE Homo sapiens
80000
-
isoenzyme M-GMD, dimeric form, gel filtration Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Homo sapiens key step in the de novo pathway of GDP-L-fucose biosynthesis ?
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens O60547
-
-

Purification (Commentary)

Purification (Comment) Organism
only isoenzyme L-GMD and isoenzyme M-GMD are soluble, isoenzyme S-GMD is inactive and forms a precipitate Homo sapiens

Storage Stability

Storage Stability Organism
4°C, presence of reducing agents, 36 h, 50% loss of activity Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
GDP-mannose
-
Homo sapiens GDP-4-dehydro-6-deoxy-D-mannose + H2O
-
?
additional information key step in the de novo pathway of GDP-L-fucose biosynthesis Homo sapiens ?
-
?

Subunits

Subunits Comment Organism
dimer L-GMD and M-GMD form a homodimer, M-GMD: 2 * 40200, SDS-PAGE Homo sapiens

Synonyms

Synonyms Comment Organism
L-GMD isoenzyme Homo sapiens
M-GMD isoenzyme Homo sapiens
S-GMD isoenzyme Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.8
-
GDP-mannose pH 8, M-GMD and L-GMD Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
Homo sapiens

Cofactor

Cofactor Comment Organism Structure
NADP+ tightly bound, 1 mol per homodimer Homo sapiens

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0113
-
GDP-L-fucose pH 8 Homo sapiens