Cloned (Comment) | Organism |
---|---|
L-GMD, S-GMD and M-GMD, expressed in Escherichia coli | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
GDP | competitive | Homo sapiens | |
GDP-L-fucose | non-competitive | Homo sapiens | |
GDP-L-glucose | competitive | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0035 | - |
GDP-mannose | pH 8, isoenzyme M-GMD | Homo sapiens | |
0.00364 | - |
GDP-mannose | pH 8, isoenzyme L-GMD | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
38700 | - |
isoenzyme S-GMD, SDS-PAGE | Homo sapiens |
40200 | - |
isoenzyme M-GMD, SDS-PAGE | Homo sapiens |
42000 | - |
isoenzyme L-GMD, SDS-PAGE | Homo sapiens |
80000 | - |
isoenzyme M-GMD, dimeric form, gel filtration | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Homo sapiens | key step in the de novo pathway of GDP-L-fucose biosynthesis | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | O60547 | - |
- |
Purification (Comment) | Organism |
---|---|
only isoenzyme L-GMD and isoenzyme M-GMD are soluble, isoenzyme S-GMD is inactive and forms a precipitate | Homo sapiens |
Storage Stability | Organism |
---|---|
4°C, presence of reducing agents, 36 h, 50% loss of activity | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
GDP-mannose | - |
Homo sapiens | GDP-4-dehydro-6-deoxy-D-mannose + H2O | - |
? | |
additional information | key step in the de novo pathway of GDP-L-fucose biosynthesis | Homo sapiens | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | L-GMD and M-GMD form a homodimer, M-GMD: 2 * 40200, SDS-PAGE | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
L-GMD | isoenzyme | Homo sapiens |
M-GMD | isoenzyme | Homo sapiens |
S-GMD | isoenzyme | Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.8 | - |
GDP-mannose | pH 8, M-GMD and L-GMD | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | tightly bound, 1 mol per homodimer | Homo sapiens |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0113 | - |
GDP-L-fucose | pH 8 | Homo sapiens |