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Literature summary for 4.2.1.47 extracted from
- Expression, purification and characterization of GDP-D-mannose 4,6,-dehydratase from Escherichia coli (1997), FEBS Lett., 412, 126-130.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| as fusion protein | Escherichia coli |
General Stability
| General Stability | Organism |
|---|---|
| very unstable | Escherichia coli |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| GDP | no inhibition | Escherichia coli |  |
| GDP-D-glucose | up to 34% inhibition | Escherichia coli | |
| GDP-L-fucose | up to 73% inhibition | Escherichia coli | |
| GDP-L-fucose | - |
Klebsiella pneumoniae | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.222 | - |
GDPmannose | determined with fusion protein | Escherichia coli | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | activates | Escherichia coli | |
| Mg2+ | activates | Escherichia coli | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 42000 | - |
6 * 42000, SDS-PAGE | Escherichia coli |
| 250000 | - |
gel filtration | Escherichia coli |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| GDPmannose | Escherichia coli | - |
? | - |
? | |
| GDPmannose | Klebsiella pneumoniae | - |
? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
| Klebsiella pneumoniae | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Escherichia coli |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 2.3 | - |
determined with fusion protein | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| GDP-mannose | - |
Escherichia coli | GDP-4-dehydro-6-deoxy-D-mannose + H2O | - |
? | |
| GDP-mannose | - |
Klebsiella pneumoniae | GDP-4-dehydro-6-deoxy-D-mannose + H2O | - |
? | |
| GDPmannose | - |
Escherichia coli | ? | - |
? | |
| GDPmannose | - |
Klebsiella pneumoniae | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| hexamer | 6 * 42000, SDS-PAGE | Escherichia coli |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
- |
Escherichia coli |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADP+ | - |
Escherichia coli | |
| NADP+ | - |
Klebsiella pneumoniae | |
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