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Literature summary for 4.2.1.47 extracted from

  • Broschat, K.O.; Chang, S.; Serif, G.
    Purification and characterization of GDP-D-mannose 4,6 dehydratase from porcine thyroid (1985), Eur. J. Biochem., 153, 397-401.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
GDP 27% inhibition Sus scrofa
GDP-D-glucose 35% inhibition Sus scrofa
GDP-L-fucose 2% inhibition Sus scrofa
GTP 58% inhibition Sus scrofa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0038
-
GDPmannose
-
Sus scrofa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
41500
-
6 * 41500, SDS-PAGE Sus scrofa
251000
-
sedimentation equilibrium centrifugation Sus scrofa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
GDPmannose Sus scrofa
-
?
-
?

Organism

Organism UniProt Comment Textmining
Sus scrofa
-
pig
-

Purification (Commentary)

Purification (Comment) Organism
-
Sus scrofa

Source Tissue

Source Tissue Comment Organism Textmining
thyroid gland
-
Sus scrofa
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.036
-
-
Sus scrofa

Storage Stability

Storage Stability Organism
-20¦C, 2 M sucrose, 4 months Sus scrofa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
GDP-mannose
-
Sus scrofa GDP-4-dehydro-6-deoxy-D-mannose + H2O
-
?
GDPmannose
-
Sus scrofa ?
-
?

Subunits

Subunits Comment Organism
hexamer 6 * 41500, SDS-PAGE Sus scrofa

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Sus scrofa

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
-
-
unstable to freezing except at high sucrose concentration Sus scrofa
4
-
stable for 1 day, 50% loss of activity Sus scrofa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.1
-
-
Sus scrofa

pH Range

pH Minimum pH Maximum Comment Organism
6.5 8
-
Sus scrofa